초록 ▼

A partially purified proteinaceous bone-inducing factor of 10,000 to 30,000 daltons is described. It is derived from demineralized bovine bone by extraction with a chaotropic agent, gel filtration, cation exchange chromatography using carboxymethyl cellulose at pH 4.8 and gradient elution with NaCl

A partially purified proteinaceous bone-inducing factor of 10,000 to 30,000 daltons is described. It is derived from demineralized bovine bone by extraction with a chaotropic agent, gel filtration, cation exchange chromatography using carboxymethyl cellulose at pH 4.8 and gradient elution with NaCl at 10 mM to about 150 mM.

대표청구항 ▼

A process for preparing a partially purified bone-inducing factor which comprises: (a) treating demineralized bone with a chaotropic extractant that solubilizes nonfibrous proteins; (b) subjecting the extract from step (a) to gel filtration to recover a fraction containing proteins of molecular weig

A process for preparing a partially purified bone-inducing factor which comprises: (a) treating demineralized bone with a chaotropic extractant that solubilizes nonfibrous proteins; (b) subjecting the extract from step (a) to gel filtration to recover a fraction containing proteins of molecular weight 10,000-30,000; (c) adsorbing the fraction from step (b) onto a carboxymethyl cellulose cation exchanger at approximately pH 4.5-5.5; and (d) eluting an osteogenically active fraction from the cation exchanger with a sodium chloride gradient of about 10 mM to about 150 mM in the presence of a nonionic chaotropic agent.