초록 ▼

A method of immunoassay of an antigen in a liquid sample wherein a complex is formed between antigen contained in the said sample and two or more antibody reagents, and the said complex is bound to a solid support by non-covalent bonding as defined herein; and the amount of complex becoming bound to

A method of immunoassay of an antigen in a liquid sample wherein a complex is formed between antigen contained in the said sample and two or more antibody reagents, and the said complex is bound to a solid support by non-covalent bonding as defined herein; and the amount of complex becoming bound to the support is determined; the process employing at least one monoclonal antibody reagent. Labelling methods including radio-active, fluorimetric and enzyme labelling may be used to effect determination of the binding of the complex to the solid support. The solid support may take the form of particles, beads, wall-coatings on the reaction vessel or an insert of large surface area. The method is particularly applicable to the assay of TSH, CEA, HCG, alphafeto protein, immunoglobulins, viruses, allergens, bacteria, toxins, drugs and vitamins. Use of monoclonal reagents improves the specificity of the process, and also decreases non-specific binding.

대표청구항 ▼

A method of immunoassay of an antigen having multiple epitopes in a liquid sample which comprises incubating a mixture of: (a) the liquid sample, (b) labelled antibodies to the antigen under assay, (c) a reagent comprising antibodies to the antigen under assay conjugated with a reagent Z, said reage

A method of immunoassay of an antigen having multiple epitopes in a liquid sample which comprises incubating a mixture of: (a) the liquid sample, (b) labelled antibodies to the antigen under assay, (c) a reagent comprising antibodies to the antigen under assay conjugated with a reagent Z, said reagent Z being a hapten or antigenic substance, and, (d) an antibody reagent raised to reagent Z which selectively interacts with reagent Z by non-covalent bonding, but which is not directly bindable to either component (a) or component (b) the said reagent (d) being bound to a solid phase support and at least said components (b) and (c) comprising monoclonal antibodies; separating the solids fraction from the liquid fraction, determining the amount of label in one of the said fractions and, therefrom the amount of antigen present in the sample.