초록 ▼

A homogeneous specific binding assay device, a method for its preparation, and a method for its use in determining a ligand, such as antigen, hapten, or antibody, in, or the ligand binding capacity of, a liquid sample. The test device comprises a solid carrier member, such as a fibrous web matrix, e

A homogeneous specific binding assay device, a method for its preparation, and a method for its use in determining a ligand, such as antigen, hapten, or antibody, in, or the ligand binding capacity of, a liquid sample. The test device comprises a solid carrier member, such as a fibrous web matrix, e.g., paper, or a polymeric film or gel, incorporated with reagents for a homogeneous specific binding assay system which produces a detectable response, usually an electromagnetic radiation signal, that is a function of the presence or amount of the ligand in or the ligand binding capacity of the sample. Useful homogeneous specific binding assay systems include those involving enzyme substrate labels, enzyme prosthetic group labels, and enzyme labels. The detectable response preferably is a luminescent, fluorescent, spectrophotometric, or colorimetric response, which is measurable by visual observation or instrument means.

대표청구항 ▼

A test device for determining a ligand in a liquid sample based on competitive protein binding, the test device comprising (a) a first solid matrix layer element incorporated with an antibody or other naturally occurring binding protein for said ligand; and (b) a second solid matrix layer element in

A test device for determining a ligand in a liquid sample based on competitive protein binding, the test device comprising (a) a first solid matrix layer element incorporated with an antibody or other naturally occurring binding protein for said ligand; and (b) a second solid matrix layer element incorporated with a labeled reagent comprising said ligand, or a binding analog thereof, chemically coupled with a label which is detectable by an electromagnetic signal that has a wavelength between about 200 and about 900 nanometers and is distinguishable when the labeled reagent is bound by the antibody or said other binding protein compared to when it is not so bound, the first and second layer elements each having a dry thickness of between about 5 and about 100 microns and being in laminar contact such that the liquid sample is capable of passing between superposed surfaces of such layer elements.