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Methods are provided for the use of magnetically responsive particles in systems in which the separation of certain molecules, macromolecules and cells from the surrounding medium is desirable. The magnetically responsive particles may be coupled to a wide variety of molecules. The magnetic particle

Methods are provided for the use of magnetically responsive particles in systems in which the separation of certain molecules, macromolecules and cells from the surrounding medium is desirable. The magnetically responsive particles may be coupled to a wide variety of molecules. The magnetic particles can be dispersed in aqueous media without rapid settling and conveniently reclaimed from media with a magnetic field. Preferred particles do not become magnetic after application of a magnetic field and can be redispersed and reused.

대표청구항 ▼

A method for carrying out nucleic acid hybridization which comprises: (a) contacting a first nucleic acid molecule covalently coupled to a magnetically-responsive particle with a solution or suspension containing a complementary nucleic acid molecule in a reaction vessel; (b) allowing the first nucl

A method for carrying out nucleic acid hybridization which comprises: (a) contacting a first nucleic acid molecule covalently coupled to a magnetically-responsive particle with a solution or suspension containing a complementary nucleic acid molecule in a reaction vessel; (b) allowing the first nucleic acid molecule and the complementary nucleic acid molecules to hybridize; (c) magnetically separating the magnetically responsive particles with complementary nucleic acid molecules bound thereto from the solution or suspension; and (d) recovering the bound nucleic acid molecule from the magnetically-responsive particles, wherein the magnetically-responsive particle of step (a) comprises a magnetic metal oxide core generally surrounded by a coat of polymeric silane, a mass of such uncoupled particles being dispersable in aqueous media to form an aqueous dispersion having (i) a fifty-percent-turbidity-decrease settling time of greater than about 1.5 hours in the absence of a magnetic field, the magnetic field, and (ii) a ninety-five-percent-turbidity-decrease separation time of less than about 10 minutes in the presence of a magnetic field, the magnetic field being applied to the aqueous dispersion by bringing a vessel containing a volume of the dispersion into contact with a pole face of a permanent magnet, the permanent magnet having a volume which is less than the volume of the aqueous dispersion in the vessel.