초록 ▼

A method for rapid detection of microorganisms in a body fluid sample includes detecting the microorganisms after treatment of the sample with a lysing agent in order to dissolve sample components other than microorganisms, and staining with a fluorescent dye. The lysing agent may be part of a compo

A method for rapid detection of microorganisms in a body fluid sample includes detecting the microorganisms after treatment of the sample with a lysing agent in order to dissolve sample components other than microorganisms, and staining with a fluorescent dye. The lysing agent may be part of a composition which includes a culture medium thereby providing simultaneous lysis and growth before staining. Detection is preferably accomplished by reliance on the fluorescence emitted by the dye having been properly excited by light energy. A composition suitable for use in the above-described method includes a growth medium and a lysing agent.

대표청구항 ▼

A method for the detection of microorganisms in a body fluid sample comprising mixing said body fluid sample with an enzyme in a buffer whereby components of said body fluid but substantially none of the microorganisms in said sample are lysed, staining said microorganisms with ethidium bromide and

A method for the detection of microorganisms in a body fluid sample comprising mixing said body fluid sample with an enzyme in a buffer whereby components of said body fluid but substantially none of the microorganisms in said sample are lysed, staining said microorganisms with ethidium bromide and determining the presence of said microorganisms by detecting the fluorescence emitted by said ethidium bromide. A method for the detection of microorganisms in a body fluid sample comprising the steps of: (a) mixing the body fluid sample with 5XLA lysing agent to provide a mixture wherein components of said body fluid but substantially none of the microorganisms are lysed; (b) incubating said mixture; (c) adding a staining buffer and ethidium bromide to said mixture; and (d) flowing said mixture through a fluorescence activated flow cytometer and detecting the fluorescence associated with the microorganisms in said mixture.