초록 ▼

A chemically active membrane having a large surface area is provided in which a hydrophilic, microporous, skinless, polyamide membrane is chemically bound to a residue of an activating agent which is capable of reacting with a biologically active material. The chemically active membrane, formed by r

A chemically active membrane having a large surface area is provided in which a hydrophilic, microporous, skinless, polyamide membrane is chemically bound to a residue of an activating agent which is capable of reacting with a biologically active material. The chemically active membrane, formed by reacting a hydrophilic, microporous, skinless, polyamide membrane with an activating agent may be used to prepare a biologically active membrane having a large surface area which comprises an acceptor molecule such as a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, a carrier protein, a receptor, heparin, a coagulation factor, or a histone covalently bound to the hydrophilic, microporous, skinless, polyamide membrane by reacting the chemically activate membrane with the acceptor molecule.

대표청구항 ▼

A method of preparing a biologically active membrane having a large surface area comprising the steps of: reacting a hydrophilic, microporous, skinless polyamide membrane with an activating agent to form a chemically activated membrane, and reacting said chemically activated membrane with an accepto

A method of preparing a biologically active membrane having a large surface area comprising the steps of: reacting a hydrophilic, microporous, skinless polyamide membrane with an activating agent to form a chemically activated membrane, and reacting said chemically activated membrane with an acceptor molecule to form a biologically active membrane, said acceptor molecule being a member selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulin, a carrier protein, a receptor, heparin, a coagulation factor, and a histone. A method for recovering a ligand from a solution including passing a ligand solution through a biologically active membrane comprising an acceptor molecule selected from the group consisting of a monoclonal antibody, a polyclonal anitbody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydrate, an enzyme substrate, a cofactor, an inhibitor, a hormone, an IgG class of immunoglobulins, a carrier protein, a receptor, heparin, a coagulation factor, and a histone covalently bound to a hydrophilic, microporous, skinless polyamide membrane and binding said ligand to said biologically active membrane.