초록 ▼

A method of forming a therapeutic or diagnostic agent labeled with a radioactive metal ion, which comprises: contacting an unlabeled therapeutic or diagnostic agent, consisting of a substantially non-metal chelating portion and a chelating portion capable of chelating with the radioactive metal ion,

A method of forming a therapeutic or diagnostic agent labeled with a radioactive metal ion, which comprises: contacting an unlabeled therapeutic or diagnostic agent, consisting of a substantially non-metal chelating portion and a chelating portion capable of chelating with the radioactive metal ion, with an ion transfer material having the radioactive metal ion bound thereto and having a binding affinity for the radioactive metal less than the binding affinity of the chelating portion for the radioactive metal ion, wherein prior to contacting the chelating portion is unchelated or is chelated with a second metal having a binding affinity with the chelating portion less than the binding affinity of the radioactive metal ion, whereby a radiolabeled therapeutic or diagnostic agent is formed by the contacting, and separating the radiolabeled therapeutic or diagnostic agent from the ion transfer material, is disclosed along with various components and kits useful in practicing this method and several variations thereof.

대표청구항 ▼

A radioisotopicassay for detecting an analyte in a sample, which comprises: (1) forming a diagnostic agent labeled with a radioactive metal ion by contacting (i) an unlabeled diagnostic agent comprising (a) a molecularly recognizable portion attached to (b) a chelating portion capable of substantial

A radioisotopicassay for detecting an analyte in a sample, which comprises: (1) forming a diagnostic agent labeled with a radioactive metal ion by contacting (i) an unlabeled diagnostic agent comprising (a) a molecularly recognizable portion attached to (b) a chelating portion capable of substantially chelating with said radioactive metal ion, wherein said chelating portion is not a part of said molecularly recognizable portion, with (ii) an ion transfer material having said radioactive metal ion bound thereto and having a binding affinity for said radioactive metal ion less than the binding affinity of said chelating portion for said radioactive metal ion, wherein, prior to said contacting, said chelating portion is unchelated or is chelated with a second metal having a binding affinity with said chelating portion less than the binding affinity of said radioactive metal ion, whereby a radiolabeled diagnostic agent is formed by said contacting; and (2) contacting the sample to be detected when the diagnostic agent so-formed to provide a reaction mixture; (3) separating, if necessary, said ion transfer material and any diagnostic agent which has not reacted with the analyte; and (4) detecting any signal generated from diagnostic agent which has so-reacted.