초록 ▼

Dry chemistry reagent system, kit and method for detection of an analyte such as glucose, cholesterol, urea, antigen or antibody. The reagent system is a porous membrane or bibulous film having a porosity gradient from one planar surface to the other. In the membrane are uniformly distributed an ind

Dry chemistry reagent system, kit and method for detection of an analyte such as glucose, cholesterol, urea, antigen or antibody. The reagent system is a porous membrane or bibulous film having a porosity gradient from one planar surface to the other. In the membrane are uniformly distributed an indicator, a flow control agent and a reagent cocktail. The membrane is also modified by a conditioning agent.

대표청구항 ▼

A rapid analytical method for screening a heterogenous fluid sample for an analyte of interest, comprising: (a) providing a dry chemistry reagent system comprising a porous membrane of essentially uniform composition and a porosity gradient from one planar surface thereof to the other, wherein said

A rapid analytical method for screening a heterogenous fluid sample for an analyte of interest, comprising: (a) providing a dry chemistry reagent system comprising a porous membrane of essentially uniform composition and a porosity gradient from one planar surface thereof to the other, wherein said porouse membrane\s inherent fluid absorption characteristics have been modified by sequentially imbibing a conditioning agent, an indicator, flow control agent and reagent cocktail into its matrix, the effect of such absorption being to effect an essentially uniform distribution of indicator, flow control agent and reagent cocktail within the porous membrane thereby enhancing the uniformity of internal structure of said porous membrane so as to modulate both the rate and the uniformity of absorption of the fluid sample and its interaction with the reagent cocktail; (b) applying a heterogenous fluid sample to the sample receptive surface of the bibulous medium of the reagent system; and (c) monitoring the surface of the bibulous medium opposite to the sample receptive surface for a change of color or fluorescence or for the development of color or fluorescence.