[특허]Electromicroinjection of particles into living cells

Electromicroinjection of particles into living cells 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	B01D-057/02 A61N-001/30 A61B-017/36 C12N-015/00
출원번호	US-0853823 (1986-04-18)
발명자 / 주소	Ray F. Andrew (Los Alamos NM) Cram L. Scott (Los Alamos NM) Galey William R. (Albuquerque NM)
출원인 / 주소	The United States Department of Energy (Washington DC 06)
인용정보	피인용 횟수 : 72 인용 특허 : 0

초록 ▼

Method and apparatus for introducing particles into living cells. Fluorescently-stained human chromosomes are introduced into cultured, mitotic Chinese hamster cells using electromicroinjection. The recipient cells frequently survived the physiological perturbation imposed by a successful chromosome injection. Successfully injected recipient cells maintained viability as evidenced by their ability to be expanded. The technique relies on the surface charge of fluorescently stained chromosomes and their ability to be attracted and repelled to and from the tip of a micropipette. The apparatus includes a micropipette having a tip suitable for piercing the membrane of a target cell and an electrode inserted into the lumen thereof. The target cells and suspended particles are located in an electrically conducted solution, and the lumen of the micropipette is filled with an electrically conducting solution which contacts the electrode located therein. A second electrode is also located in the conducting solution containing the target cells and particles. Voltages applied to the electrode within the micropipette attract the particles to the region of the tip thereof. The particles adhere to the surface of the micropipette with sufficient force that insertion of the micropipette tip and attached particle through the membrane of a target cell will not dislodge the particle. By applying a voltage having the opposite polarity of the attraction voltage, the particles are expelled from the micropipette to which is then withdrawn from the cell body.

대표청구항 ▼

An apparatus for introducing individual particles suspended in an electrically conducting aqueous solution into recipient biological cells. the apparatus comprising in combination: an injection micropipette having an exit orifice size for piercing the membrane of a target recipient cell and an entrance orifice; an electrically conducting medium located within the lumen of said injection micropipette in contact with the exit orifice thereof; first electrode means located within the lumen of said injection micropipette, said first electrode means having a first end positioned within said electrically conducting medium contained in the lumen of said injection micropipette, and a second end which emerges from the entrance orifice thereof; second electrode means located in the electrically conducting aqueous solution containing the recipient biological cells; and means for applying a first voltage and a subsequent second voltage between the second end of said first electrode means and said second electrode means both when the exit orifice of said injection micropipette is located in the conducting aqueous solution and when the exit orifice of said injection micropipette is located within the body of a recipient cell located in the conducting aqueous solution, the first voltage having a chosen magnitude and polarity for electrostatically attracting at least one particle to the region of the exit orifice of said injection micropipette, and for holding the particle in this location during the process of piercing the membrane of a cell with the exit orifice of said injection micropipette and insertion of the particle into the recipient cell body. and the second voltage having a polarity opposite to that of the first voltage and a chosen magnitude for electrostatically repelling the particle away from the injection micropipette.

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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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연합인증

Electromicroinjection of particles into living cells 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

이 특허를 인용한 특허 (72)

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Electromicroinjection of particles into living cells 원문보기

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대표청구항 ▼

연구과제 타임라인

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이 특허를 인용한 특허 (72)

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