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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0093482 (1987-09-04) |
발명자 / 주소 |
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출원인 / 주소 |
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인용정보 | 피인용 횟수 : 450 인용 특허 : 19 |
A spectrophotometric method is described of quantitatively determining the concentration of a dilute component in either a clear or a strongly light-scattering environment containing same in unknown concentration together with a reference component of known concentration, by a series of contemporane
A spectrophotometric method is described of quantitatively determining the concentration of a dilute component in either a clear or a strongly light-scattering environment containing same in unknown concentration together with a reference component of known concentration, by a series of contemporaneous radiation-directing and measurement steps of radiation of selected varying wavelengths. Specific applications are disclosed involving the in situ, in vivo, non-invasive spectrophotometric determination of blood-borne as well as tissue species, e.g., hemoglobin, and oxyhemoglobin, and intra-cellular enzyme cytochrome c oxidase, in human body parts such as fingers, hands, toes, feet, earlobes, etc., as well as organs such as the brain, skeletal muscle, liver, etc.
A spectrophotometric method of quantitatively determining the concentration of a dilute component in an environment containing the dilute component of known identity but of unknown concentration in combination with a reference component of known concentration, by a series of successive, substantiall
A spectrophotometric method of quantitatively determining the concentration of a dilute component in an environment containing the dilute component of known identity but of unknown concentration in combination with a reference component of known concentration, by a series of successive, substantially contemporaneous measurements of transmitted and/or reflected radiation at selected wavelengths, comprising: (a) determining the apparent effective pathlength in said environment; (b) directing at said environment incident electromagnetic radiation of a first wavelength in a selected spectral region at which the dilute and/or reference component(s) exhibit absorption for the electromagnetic radiation; (c) measuring the first wavelength radiation transmitted and/or reflected by the environment; (d) directing at the environment incident electromagnetic radiation of at least one other wavelength in the selected spectral region at which the dilute and/or reference component(s) exhibit absorption of different relative intensities than for the first wavelength incident radiation; (e) measuring the other wavelength radiation transmitted and/or reflected by the environment; (f) determining extinction coefficient values for the dilute component at said first and other wavelengths in said environment; and (g) based on the apparent effective pathlength determined for the environment, the extinction coefficient values for the dilute component at said first, and other wavelengths, and the measured absorbed and/or reflected radiation at said wavelengths, determining the relative amount of the dilute component to the amount of the reference component, as the concentration of the dilute component in the environment.
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