초록 ▼

A system and method is disclosed for the rapid detection and (or) purification of analytes in a sample. The system makes use of a fluid receptacle containing a capture reagent and a manifold having two ports. A sample containing an analyte disposed within the fluid receptacle is subjected to a captu

A system and method is disclosed for the rapid detection and (or) purification of analytes in a sample. The system makes use of a fluid receptacle containing a capture reagent and a manifold having two ports. A sample containing an analyte disposed within the fluid receptacle is subjected to a capture reagent and processed by inserting the open end of the receptacle in one port. Vacuum, gas, reagents, and wash fluids are then applied to the second port to effect rapid interaction between the capture reagent and analyte, efficient washing of the capture reagent and detection and/or collection of the analyte. The system and method provide a rapid safe, uncomplicated means of automating processes based on affinity complexation principles. Encompassed within this category of processes are immunoassays, nucleic acid hybridization tests and affinity separations.

대표청구항 ▼

An article of manufacture for processing test liquids having analytes comprising a closed, one piece container having an access port, the container being shaped to have a bulbous portion and an elongated passageway providing a path of two way liquid communication between the bulbous portion and the

An article of manufacture for processing test liquids having analytes comprising a closed, one piece container having an access port, the container being shaped to have a bulbous portion and an elongated passageway providing a path of two way liquid communication between the bulbous portion and the access port, a first porous retainer positioned in the passageway adjacent the bulbous portion and occupying only a portion of the passageway such that the volume of the passageway between the retainer and the access port exceeds that of the test liquids, a plurality of solid support beads, having a capture reagent for use in complexation reactions and affinity separations, immobilized on the surface thereof, disposed in the bulbous portion of the container, the passageway and the bulbous portion each defining volumes, the passageway volume being less than the bulbous portion volume, the porous retainer having pores large enough not to restrict the flow of liquids, cells, microorganisms, cellular fragments or particulates of interest for analysis and small enough to prevent the passage of the beads from the bulbous portion into the passageway, and the bulbous portion of the container being collapsible, whereby test liquids may be passed back and forth through the retainer and about the support beads to achieve improved capture efficiency.