초록 ▼

Detection or measurement of chemical indicator paramaters such as pH or of the concentration reactants of chemical reactions is accomplished by employing at least two fluorescent species with predominant emission at different optical wavelength regions, such that a synthesized fluorescence color cha

Detection or measurement of chemical indicator paramaters such as pH or of the concentration reactants of chemical reactions is accomplished by employing at least two fluorescent species with predominant emission at different optical wavelength regions, such that a synthesized fluorescence color change is created. In this manner, measurement is improved and simple visual inspection can reveal whether or not a chemical reaction or parameter has changed significantly, while retaining the sensitivity of fluorescence measurement or detection.

대표청구항 ▼

A process for detecting a change in the concentration of a microbiologically or biochemically active material comprising: (a) providing microdroplets containing: (i) a source of the microbiologically for biochemically active material; and (ii) at least two fluorescent compounds wherein at least one

A process for detecting a change in the concentration of a microbiologically or biochemically active material comprising: (a) providing microdroplets containing: (i) a source of the microbiologically for biochemically active material; and (ii) at least two fluorescent compounds wherein at least one first fluorescent compound is capable of contributing to a change in color by increasing in fluorescent emission intensity in response to a change in concentration of the microbiologically or biochemically active material, and at least one second fluorescent compound which is a reference standard, the proportion of which fluorescent compounds changes as the concentration of the microbiologically or biochemically active material changes, thereby producing a color change in fluorescent emissions from the fluorescent compounds; (b) determining the color of the fluorescent emission from the fluorescent compounds; (c) maintaining the microdroplets under conditions which allow the concentration of the microbiologically or biochemically active material to change; and (d) detecting the change in the concentration of the microbiologically or biochemically active material by determining the change in color of the fluorescent emission from the fluorescent compounds.