초록 ▼

A method for the determination of a free protein subunit of a quaternary protein in a sample, which comprises: (a) contacting a sample with a first immunological binding partner which is or will be bound to a carrier, wherein the first immunological binding partner binds epitopic determinants bindab

A method for the determination of a free protein subunit of a quaternary protein in a sample, which comprises: (a) contacting a sample with a first immunological binding partner which is or will be bound to a carrier, wherein the first immunological binding partner binds epitopic determinants bindable only on the free protein subunit; (b) incubating the components of step (a) for a period of time and under conditions sufficient to form an immune complex between the free protein subunit, the first immunological binding partner, and the carrier; (c) separating the carrier of step (b) from the sample; (d) adding to the carrier of step (c), a detectably-labeled second immunological binding partner, wherein the second immunological binding partner binds epitopic determinants bindable on both the free protein subunit and the quaternary protein; and (e) determining the detectably-labeled second immunological binding partner in the carrier or in liquid phase.

대표청구항 ▼

A method for the determination of a free protein subunit of hCG in a sample containing intact quaternary hCG, which comprises: (a) contacting said sample with a first monoclonal antibody which is bound to a carrier, wherein said first monoclonal antibody binds epitopic determinants bindable only on

A method for the determination of a free protein subunit of hCG in a sample containing intact quaternary hCG, which comprises: (a) contacting said sample with a first monoclonal antibody which is bound to a carrier, wherein said first monoclonal antibody binds epitopic determinants bindable only on said free protein subunit; (b) incubating the components of step (a) for a period of time and under conditions sufficient to form an immune complex between said free protein subunit, said first monoclonal antibody, and said carrier; (c) separating said carrier of step (b) from said sample; (d) adding to said carrier of step (c), a detectably labeled second monoclonal antibody, wherein said second monoclonal antibody binds epitopic determinants bindable on both said free protein subunit and said intact quaternary hCG; (e) separating said carrier of step (d) from the liquid phase; and (f) determining the detectably labeled second monoclonal antibody in said carrier or in said liquid phase, which is a measure of the amount of said free protein subunit in said sample.