[특허]Method of isolating and purifying nucleic acids from biological samples

Method of isolating and purifying nucleic acids from biological samples 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C07H-021/00 C12P-019/34 C12Q-001/68 G01N-030/02
출원번호	US-0936163 (1986-12-01)
발명자 / 주소	Seligson David B. (Wauwatosa WI) Shrawder Elsie J. (San Diego CA)
출원인 / 주소	Syngene, Inc. (San Diego CA 02)
인용정보	피인용 횟수 : 130 인용 특허 : 0

초록 ▼

The method of this invention is applicable to rapid separation, isolation, and purification of DNA or RNA from biological samples. The DNA/RNA may be in double-stranded or single-stranded form. The method is particularly advantageous for resolving genetic DNA or RNA found in bacteria, virus, and mammalian cells, and for use with samples of human bodily fluids and tissues, including stool, sputum, urine, and blood samples. DNA or RNA can be separated effectively from interfering components, particularly proteins, biological pigments and mucopolysaccharides. The method of the present invention can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution.

대표청구항 ▼

The method of isolating and purifying cellular or viral nucleic acids from an aqueous solution of a biological sample containing nuclein acids together with water-soluble contamination components of lysed cells or virus selected from the group consisting of proteins, pigments, carboxylated mucopolysaccharides, sulfated mucopolysaccharides, and mixtures thereof, comprising: (a) selecting an anion exchange column material which effectively binds the target nucleic acids at a lower salt molarity than the molarity at which the target nucleic acids elute therefrom; (b) applying a sample containing nucleic acids together with water-soluble contaminating components of lysed cells or virus selected from the group consisting of proteins, pigments, carboxylated mucopolysaccharides, sulfated mucopolysaccharides, and mixtures thereof, to a column packed with the selected anion exchanger material, and said nucleic acids becoming bound to said column material; (c) washing said column with an aqueous solution of a salt at chloride molarity at which the nucleic acids remain bound to said column material, said wash molarity being close to the lowest molarity at which the nucleic acids begin to elute, said washing being of sufficient volume to wash the non-binding components through said column, including carboxylated mucopolysaccharides; (d) eluting the bound nucleic acids by passing through said column an aqueous solution of a salt having an eluting molarity corresponding to the lowest molarity at which the target nucleic acids will completely elute, said eluant being passed in sufficient volume to remove said nucleic acids from the column while leaving contaminating components including sulfated mucopolysaccharides in the column; and (e) recovering the eluted nucleic acids essentially free of mucopolysaccharides and other contaminating components.

이 특허를 인용한 특허 (130)

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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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Method of isolating and purifying nucleic acids from biological samples 원문보기

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Method of isolating and purifying nucleic acids from biological samples 원문보기

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