초록 ▼

The stability of granular carrier-bound enzymes is improved by further treating the carrier-bound enzyme with a bifunctional cross-linking agent and a polyamine. A particularly preferred cross-linking agent is glutaric dialdehyde, and a particularly preferred polyamine is polyethylene imine. Prefera

The stability of granular carrier-bound enzymes is improved by further treating the carrier-bound enzyme with a bifunctional cross-linking agent and a polyamine. A particularly preferred cross-linking agent is glutaric dialdehyde, and a particularly preferred polyamine is polyethylene imine. Preferably, the cross-linking agent is used in an amount of 5 to 250 mg per 1 gram of carrier-bound enzyme and is dissolved in a 0.1 to 10% (W/V) aqueous solution, and the polyamine is used in an amount of 3 to 240 mg per 1 gram of dry carrier and is dissolved in a 0.1 to 20% (W/V) aqueous solution.

대표청구항 ▼

A method for producing carrier-bound enzymes having improved stability comprising the steps of: (a) providing a granular carrier which has functional groups capable of forming covalent bonds and which is coated with an enzyme to produce a carrier-bound enzyme, and (b) contacting said carrier-bound e

A method for producing carrier-bound enzymes having improved stability comprising the steps of: (a) providing a granular carrier which has functional groups capable of forming covalent bonds and which is coated with an enzyme to produce a carrier-bound enzyme, and (b) contacting said carrier-bound enzyme in any sequence with an amount of 5 to 250 mg per 1 gram of dry carrier-bound enzyme of a bifunctional cross-linking agent contained in a 0.1 to 10% (w/v) aqueous solution of the cross-linking agent and with an amount of 3 to 240 mg per 1 gram of dry carrier-bound enzyme of a polyamine contained in a 0.1 to 20% (w/v) aqueous solution of said polyamine, whereby a granular carrier-bound enzyme is produced.