초록 ▼

A device for separating mononuclear cells from blood includes a collection tube, a first layer of a liquid density gradient material placed in the tube, a second layer of a gel-like substance placed in the tube and situated above the first layer, a porous foam member placed in the tube and situated

A device for separating mononuclear cells from blood includes a collection tube, a first layer of a liquid density gradient material placed in the tube, a second layer of a gel-like substance placed in the tube and situated above the first layer, a porous foam member placed in the tube and situated above and in contact with the second layer of gel-like substance, and a third layer of a Newtonian gel-like substance placed in the tube above the porous foam member. The gel-like substance of the second layer has a lower specific gravity than that of the liquid density gradient material of the first layer so that it floats on top of the first layer. The gel-like substance of the second layer becomes less viscous when heated such that it is absorbed by the porous foam member and forms a hydraulic barrier between the liquid density gradient material of the first layer and a blood sample placed in the collection tube.

대표청구항 ▼

Apparatus for separating mononuclear cells from blood, which comprises: a collection tube having a bottom closed end and an opposite open top end for receiving a blood sample, and being adapted to be centrifuged; a first layer of a liquid density gradient medium contained in the collection tube and

Apparatus for separating mononuclear cells from blood, which comprises: a collection tube having a bottom closed end and an opposite open top end for receiving a blood sample, and being adapted to be centrifuged; a first layer of a liquid density gradient medium contained in the collection tube and situated at the closed end of the tube; a second layer of a gel-like substance contained in the collection tube and placed on top of the first layer of liquid density gradient medium, the second layer having a specific gravity which is less than that of the first layer so that it floats on top of the first layer; and a porous foam member contained in the collection tube and situated on top of and in contact with the second layer of gel-like substance; the collection tube being of sufficient size to define a free space above the porous foam member to receive a blood sample; the second layer of gel-like substance being adapted to coat a portion of the porous foam member with which it is contact to form a hydraulic barrier between a blood sample received by the tube and the first layer of liquid density gradient medium to prevent intermixing of the first layer and the blood sample prior to centrifuging the tube.