초록 ▼

Liquid chromatography separations of solutes are achieved using porous hollow fibers. The pores of the hollow fibers immobilize a solute-absorbing phase (preferably organic) which has a greater absorbance affinity towards at least one solute in a mixture of solutes. By passing the solute mixture thr

Liquid chromatography separations of solutes are achieved using porous hollow fibers. The pores of the hollow fibers immobilize a solute-absorbing phase (preferably organic) which has a greater absorbance affinity towards at least one solute in a mixture of solutes. By passing the solute mixture through the central lumen of the hollow fibers, chromatographic separation are realized due to the greater retention time of that solute with which the immobilized phase has greater absorbance affinity. The immobilized phase may be a liquid organic which may contain a surfactant so as to form reversed micelles or it may be in the form of a polymeric gel. Separations of biological species (e.g., proteins) may thus be accomplished by means of the present invention.

대표청구항 ▼

A liquid chromatography method for separating at least solute species A and B comprising the steps of: (a) immobilizing, within pores of a number of open celled, microporous hollow fibers having an average pore size of less than 5,000 Å, an inner diameter of from about 5 to about 1500 microns, a coe

A liquid chromatography method for separating at least solute species A and B comprising the steps of: (a) immobilizing, within pores of a number of open celled, microporous hollow fibers having an average pore size of less than 5,000 Å, an inner diameter of from about 5 to about 1500 microns, a coefficient of variation in inner diameter of less than about 8%, and an average ratio of maximum pore density to minimum pore density around the circumference of the fiber of less than about 3:1, a solute-absorbing phase having a greater affinity for absorbing/desorbing solute species A as compared to solute species B; (b) passing a carrier liquid stream through the lumens of said microporous hollow fibers; and then (c) injecting a sample mixture comprised of solute species A and B into said carrier liquid stream so that a liquid mixture comprised of said carrier liquid and said solute species A and B passes through the lumens of said hollow fibers; (d) allowing said solute species A to be absorbed preferentially by said immobilized solute-absorbing phase so that a first mixture comprised essentially of the carrier liquid and solute species B is initially discharged from said hollow fiber lumens; and then (e) allowing the solute species A to desorb into said carrier liquid stream so as to form a second mixture comprised essentially of the carrier liquid and the solute species A which is discharged from said hollow fiber lumens subsequent to the discharge of said first mixture comprised essentially of the carrier liquid and solute species B, whereby solute species A and B are separated from said sample mixture thereof.