초록 ▼

A test strip for analysis of analytes such as antigens, antibodies or polynucleotides employs a chromatographic medium and a solvent capable of transporting reagents and/or sample. Reagents are selected and disposed on the medium such that a labeled (first) reagent arrives at the detection (third) z

A test strip for analysis of analytes such as antigens, antibodies or polynucleotides employs a chromatographic medium and a solvent capable of transporting reagents and/or sample. Reagents are selected and disposed on the medium such that a labeled (first) reagent arrives at the detection (third) zone only after analyte is immobilized there and non-reactive sample componets have been transported beyond the detection zone. This sequential arrival is accomplished by the relative mobility of the reagent or sample; or by the site relationship of the zones. Preferably, the site relationship of the sample (second) zone and the label (first) zone is such that a plurality of pathways guide the sample and labeled reagent and any subsequent reagents to the detection zone in the recited order. Single and multiple pathway devices are disclosed.

대표청구항 ▼

A test strip for analysis of an analyte in a sample by means of a sequential series of reactions, said strip comprising: a length of chromatographic material having capillarity and the capacity for chromatographic solvent transport of non-immobilized reagents and reactive sample components by means

A test strip for analysis of an analyte in a sample by means of a sequential series of reactions, said strip comprising: a length of chromatographic material having capillarity and the capacity for chromatographic solvent transport of non-immobilized reagents and reactive sample components by means of a selected chromatographic solvent, said strip including, a first end at which chromatographic solvent transport begins; a second end, at which chromatographic solvent transport ends; a plurality of chromatographic solvent transport pathways; a plurality of zones positioned between said first and second ends, said zones including, a first zone impregnated with a first reagent which is mobile in said solvent and capable of a specific binding reaction with said analyte, a second zone for receiving said sample, and a third zone, downstream of said second zone, impregnated with a second reagent which is immobilized against solvent transport and is capable of a specific binding reaction with the analyte so as to immobilize the analyte in said third zone, said first and second zones being spaced sufficiently from said first end to permit contact of said first end but not said first and second zones with said chromatographic solvent, and means for detecting said first reagent at said third zone as a measure of the analyte, whereby, after said first end is dipped into said chromatographic solvent, the relative mobility of said sample components and said first reagent is controlled by said plurality of chromatographic solvent transport pathways such that the analyte is transported to and immobilized against solvent transport at the third zone prior to the first reagent reaching the third zone, and whereby interfering sample components and non-analyte components of the sample which are reactive with said first reagent are cleared from said third zone by chromatographic solvent transport prior to the first reagent reaching said third zone.