초록 ▼

A static oxygenator for oxygenating a liquid, particularly a suspension culture of animal cells in a liquid culture medium, comprised of a bottom gassing portion comprised of generally concentric vertically oriented hollow cylinders of porous gas-permeable, liquid-impermeable material, and an upper

A static oxygenator for oxygenating a liquid, particularly a suspension culture of animal cells in a liquid culture medium, comprised of a bottom gassing portion comprised of generally concentric vertically oriented hollow cylinders of porous gas-permeable, liquid-impermeable material, and an upper degassing section comprised of vertical extensions of the concentric cylinders such that at least one liquid overflow weir is provided at the juncture between the bottom and upper sections. Gas directed into the bottom of the annular space between the concentric cylinders rises therein and, across the porous material, oxygenates liquid in contact therewith or in proximity thereto up to the point where the liquid overflows the weir, and the gas then continues up the annular space in the vertical extensions for degassing through the porous material above the liquid level.

대표청구항 ▼

A static apparatus for oxygenating a suspension culture of animal cells in a liquid culture medium, comprising a bottom portion comprising generally concentric vertically-oriented inner and outer hollow cylinders, the walls of which are composed of porous gas-permeable, liquid-permeable material, so

A static apparatus for oxygenating a suspension culture of animal cells in a liquid culture medium, comprising a bottom portion comprising generally concentric vertically-oriented inner and outer hollow cylinders, the walls of which are composed of porous gas-permeable, liquid-permeable material, so as to provide between the cylindrical surfaces of said concentric cylinders a hollow annular space adapted to receive oxygen-containing gas and transfer at least a portion of said gas substantially bubble-free across said porous material to a liquid phase in contact with or in proximity to said cylindrical surfaces of said bottom portion; and a top portion comprising vertical extensions of said concentric cylinders which are interrupted at least one area along their circumferential surfaces such that at least one liquid overflow area is defined at the base area at said interrupted surfaces, and such that a continuation of sections of said hollow annular space exists between the vertically-extended surfaces of said concentric cylinders, said continuation of said sections of said hollow annular space being adapted to receive oxygen-containing gas from said hollow annular space of said bottom portion and to release oxygen-containing gas across said porous material above said overflow areas, said apparatus being adapted to be stationarily arranged in a suspension culture vessel and unassociated with means for rotating or vibrating said apparatus.