초록 ▼

A method and device for determining the presence of an analyte in a sample suspected of containing the analyte is disclosed. The method involves contacting a test solution containing the sample, an antibody for the analyte, and a conjugate of the analyte and a label with a contact portion of a piece

A method and device for determining the presence of an analyte in a sample suspected of containing the analyte is disclosed. The method involves contacting a test solution containing the sample, an antibody for the analyte, and a conjugate of the analyte and a label with a contact portion of a piece of bibulous material capable of being traversed in at least one direction by the test solution through capillary action. The bibulous material contains a first receptor capable of binding to said conjugate. The first receptor is non-diffusively bound to a situs on the bibulous material separate from the contact portion. The bibulous material further contains a second receptor capable of binding the antibody to the analyte between the situs and the contact portion. The second receptor is non-diffusively bound to the bibulous material. At least a portion of the test solution is allowed to traverse the bibulous material by capillary action and thereby contact the situs. The situs is examined for the presence of the conjugate. To this end, the situs can be exposed to a signal producing means capable of interacting with the label to produce a signal in relation to the amount of analyte in the test solution. The signal produced at the situs is then detected.

대표청구항 ▼

A method for determining the presence of an analyte, that is capable of binding specifically to an antibody, in a sample suspected of containing said analyte, which comprises (a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a

A method for determining the presence of an analyte, that is capable of binding specifically to an antibody, in a sample suspected of containing said analyte, which comprises (a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a label, a contact portion of a piece of bibulous material capable of being traversed in at least one direction by said test solution by capillary migration, said bibulous material containing non-diffusively bound to a situs on said bibulous material separated from said contact portion a first receptor capable of binding to said conjugate, the surface area of said situs being less than that of said bibulous material, said bibulous material further containing a second receptor capable of binding said antibodies to said analyte wherein said second receptor is non-diffusively bound to said bibulous material at a portion thereof between said situs and said contact portion, (b) allowing at least a portion of said test solution to traverse said bibulous material by capillary migration and thereby contact said situs, and (c) examining said situs for the presence of said conjugate, and relating the presence of said conjugate at said situs to the presence of analyte in the sample.