초록 ▼

A 2,356 base pair fragment isolated from the human X chromosome, designated as Xrep, has been found to exert a positive effect on plasmid replication in both prokaryotic and eukaryotic cells. The Xrep, in addition, has been found to increase transcription of DNA, thus leading to increased expression

A 2,356 base pair fragment isolated from the human X chromosome, designated as Xrep, has been found to exert a positive effect on plasmid replication in both prokaryotic and eukaryotic cells. The Xrep, in addition, has been found to increase transcription of DNA, thus leading to increased expression of desired protein products. The Xrep segment has been fully sequenced and portions thereof have been found to exhibit homologies with enhancer sequences contained in various viruses.

대표청구항 ▼

An isolated DNA sequence that functions to produce increased yields of cloning and expression vectors and also increased expression of an associated gene, when introduced into a host prokaryotic, yeast, or COS-1 cell, said cell not containing a restriction enzyme that cleaves said vectors, said sequ

An isolated DNA sequence that functions to produce increased yields of cloning and expression vectors and also increased expression of an associated gene, when introduced into a host prokaryotic, yeast, or COS-1 cell, said cell not containing a restriction enzyme that cleaves said vectors, said sequence containing a functionally active portion of the DNA segment corresponding to the nucleic acid sequence shown in FIGS. 5(a), (b), and (c), joined in the order (a), (b), and (c).