초록 ▼

An improved heterogeneous immunoassay is provided which includes the utilization of support materials capable of reversible immobilization of proteinaceous binding partners of biological material of interest and the removal of labeled complexes from these supports through the use of release reagents

An improved heterogeneous immunoassay is provided which includes the utilization of support materials capable of reversible immobilization of proteinaceous binding partners of biological material of interest and the removal of labeled complexes from these supports through the use of release reagents permitting the subsequent solution phase determination of the amount of label.

대표청구항 ▼

In a heterogeneous immunoassay of this invention comprising any standard heterogeneous immunoassay wherein the improvement comprises the modification of assay steps as follows: (a) absorbing a binding partner of the analyte of interest on a solid or liquid support to permit subsequent release of sai

In a heterogeneous immunoassay of this invention comprising any standard heterogeneous immunoassay wherein the improvement comprises the modification of assay steps as follows: (a) absorbing a binding partner of the analyte of interest on a solid or liquid support to permit subsequent release of said binding partner from said support after the formation of a complex between the binding partner, said analyte; and a labeled binding partner; (b) storing said support having said binding partner absorbed thereon under conditions which preserve said releasability; (c) contacting said support having said binding partner absorbed thereon with said analyte and a labeled binding partner of the analyte to form a complex; (d) contacting the complex formed in step (c) with a release agent selected from the group consisting of monovalent salts, surfactants and organic bases; and (e) measuring the amount of label in said complex released in step (d) from said support.