초록 ▼

A method for monitoring the glucose level in a body fluid uses an apparatus which includes a conjugate of glucose oxidase with a fluorescent dye coated onto an optical fiber in contact with the body fluid, a source of excitation light and a fluorescence emission detector. Glucose is oxidized by oxyg

A method for monitoring the glucose level in a body fluid uses an apparatus which includes a conjugate of glucose oxidase with a fluorescent dye coated onto an optical fiber in contact with the body fluid, a source of excitation light and a fluorescence emission detector. Glucose is oxidized by oxygen in the body fluid causing a decrease in oxygen concentration at the enzyme. The fluorescent dye is sensitive to oxygen quenching so that, when the oxygen concentration decreases, fluorescence emission increases in direct proportion to the glucose concentration in the fluid.

대표청구항 ▼

An apparatus for monitoring glucose concentration in a body fluid comprising: (a) a first cable comprising first and second optical fibers having immobilized thereon a first conjugate comprising active glucose oxidase and a fluorescent dye, emission from said dye being sensitive to oxygen quenching;

An apparatus for monitoring glucose concentration in a body fluid comprising: (a) a first cable comprising first and second optical fibers having immobilized thereon a first conjugate comprising active glucose oxidase and a fluorescent dye, emission from said dye being sensitive to oxygen quenching; (b) a second cable comprising third and fourth optical fibers having immobilized thereon a second conjugate comprising inactive glucose oxidase and the same aforementioned fluorescent dye; (c) a light source in excitation light transmissive communication with said first conjugate through said first fiber and with said second conjugate through said third fiber; and (d) a detector in fluorescence emission transmissive communication with said first conjugate through said second fiber and with said second conjugate through said fourth fiber.