초록 ▼

A method and apparatus is disclosed for the genetic transformation of soybean plants and plant lines by particle mediated transformation. Foreign genes are introduced into regenerable soybean tissues by coating on carrier particles which are physically accelerated into plant tissues. The treated pla

A method and apparatus is disclosed for the genetic transformation of soybean plants and plant lines by particle mediated transformation. Foreign genes are introduced into regenerable soybean tissues by coating on carrier particles which are physically accelerated into plant tissues. The treated plant tissues are then recovered and regenerated into whole sexually mature plants. The progeny are recovered from seed set by these plants and a portion of these progeny will contain in their genome the foreign gene. The procedure may be used to create novel genetically engineered soybean plants and lines.

대표청구항 ▼

A method of making a genetically transformed soybean plant comprising the steps of: preparing copies of a foreign gene including a coding region and flanking regulatory sequences effective to express the coding region in soybean cells; joining copies of the foreign gene to biologically inert carrier

A method of making a genetically transformed soybean plant comprising the steps of: preparing copies of a foreign gene including a coding region and flanking regulatory sequences effective to express the coding region in soybean cells; joining copies of the foreign gene to biologically inert carrier particles; placing an embryonic axis from a soybean seed on a target surface; physically accelerating the particles carrying the foreign gene copies at the target surface in such a fashion that some particles lodge in the interior of at least some of the cells of the soybean embryonic axis; inducing shoot formation directly from the embryonic axis by hormone treatment without intermediate proliferation of tissue in callus culture; cultivating the shoot into a whole sexually mature soybean plant; and verifying the existence of the foreign gene in the tissues of the regenerated plant.