초록 ▼

The present invention relates to a method of obtaining, on a large scale by tissue culture, tuber of potato, corm of taro, konjak, jack-in-the-culprit, rhizome of Scopolia japonica, tuberous root of Chinese yam, etc. having an enough large size to cultivate seed tubers by one cultivation in the soil

The present invention relates to a method of obtaining, on a large scale by tissue culture, tuber of potato, corm of taro, konjak, jack-in-the-culprit, rhizome of Scopolia japonica, tuberous root of Chinese yam, etc. having an enough large size to cultivate seed tubers by one cultivation in the soil. This method comprises culturing the plant itself or its slices in a liquid medium till the plant grows to a size of 15 to 30 cm on the average, sinking 90% or more of the plant in the liquid medium, while further performing cultivation, reducing the amount of the liquid medium with passage of time to such a degree that 50 to 98% of the plant is exposed into the aerial phase to thereby obtain a large amount of tuber, corm, rhizome or tuberous root in a short time.

대표청구항 ▼

A method for in vitro propagation of potato plants, of forcing such plants and of inducing propagules to form mini-tubers of enhanced size, the methods including the steps of: transplanting a potato plant initiate, the length of which does not exceed 10 cm, said plant initiate having at least a bud,

A method for in vitro propagation of potato plants, of forcing such plants and of inducing propagules to form mini-tubers of enhanced size, the methods including the steps of: transplanting a potato plant initiate, the length of which does not exceed 10 cm, said plant initiate having at least a bud, said plant initiate obtained by culture of potato plant material in a first sparged liquid medium of about 3% sucrose content; culturing the plant initiate under an illuminance of 200 to 10,000 lux until an average length thereof reaches a length of 15 to 30 cm; transferring the resulting plant to a second sparged liquid medium, said second sparged liquid medium containing about 9% sucrose; maintaining a quantity of the second sparged liquid medium sufficient to cover at least 90% of the resulting plant for a preselected period of time; and, while further performing culture, inducing the formation of mini-tubers by reducing illuminance to not greater than 200 lux, and decreasing said second sparged liquid medium with passage of time until 50 to 98% of the plant is exposed to the atmosphere, until at least one mini-tuber is formed.