초록 ▼

System for quantifying components in a liquid sample, for example white blood cells in blood, based on use of substantially non-compressible beads of uniform diameter as spacers between a microscope slide and a cover slip. The distance between the slide and cover slip is determined by the diameter o

System for quantifying components in a liquid sample, for example white blood cells in blood, based on use of substantially non-compressible beads of uniform diameter as spacers between a microscope slide and a cover slip. The distance between the slide and cover slip is determined by the diameter of the beads, and thus the volume of the sample being viewed can be precisely determined. Prior to use, the beads may be adhered to a microscope slide or stirring rod in a dried adhesive matrix which is soluble in the liquid sample to be examined.

대표청구항 ▼

A method for quantifying components in a liquid sample, comprising: placing a liquid sample on a dried adhesive matrix ona microscope slide, said matrix being soluble in the liquid sample and having located therein a plurality of substantially spherical beads of uniform diameter made of a substantia

A method for quantifying components in a liquid sample, comprising: placing a liquid sample on a dried adhesive matrix ona microscope slide, said matrix being soluble in the liquid sample and having located therein a plurality of substantially spherical beads of uniform diameter made of a substantially non-compressible material; mixing the liquid sample and matrix with a stirring instrument so tht thematrix dissolves inthe liqiud sample; plcing a cover slip over the sample and beads so that the cover slip rests on the beads and is spaced from the slide by a known distance determined by the diameter of the beads; viewing the sample disposed between the slide and cover slip under magnification with a microscope; and counting the components in a known volume of the sample, wherein the volume is determined by measuring the viewing area of the microscope and multiplying the viewing area by the distance between the slideand cover slip as determined by the diameter of the beads.