|국가/구분||United States(US) Patent 등록|
|미국특허분류(USC)||435/30 ; 435/5 ; 435/29 ; 435/34|
|발명자 / 주소|
|출원인 / 주소|
|인용정보||피인용 횟수 : 22 인용 특허 : 0|
A bacterial staining composition, process and apparatus for detection and quantitation of gram negative and gram positive bacteria in fluid samples are disclosed. The composition comprises a net negatively charged filter onto which bacteria are adsorbed and concentrated, an acid diluent which alters the electrostatic charge of the bacteria, thus enabling adsorption of the bacteria to the filter, a bacterial stain operative at about pH 8 to 12, and a washing reagent which enhances the diffusion of free dye away from the immobilized, stained bacterial. The...
A process for concentrating immobilizing and staining bacteria contained within a fluid sample which bacteria normally possesses a net negative charge, comprising: (a) diluting a sample fluid with an inorganic acid having a pH in the range of from 1 to 3 to convert said negatively charged bacteria to a net positive charge; (b) electrostatically adsorbing said converted net positively charged bacteria in a fluid sample to a designated portion of a net negatively charged filter having a pore size exceeding the size of said bacteria for electrostatic adsorp...