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"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"

특허 상세정보

Method and apparatus for detection and quantitation of bacteria

국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) C12Q-001/24    C12M-001/34   
미국특허분류(USC) 435/30 ; 435/5 ; 435/29 ; 435/34
출원번호 US-0427920 (1989-10-25)
발명자 / 주소
출원인 / 주소
인용정보 피인용 횟수 : 22  인용 특허 : 0
초록

A bacterial staining composition, process and apparatus for detection and quantitation of gram negative and gram positive bacteria in fluid samples are disclosed. The composition comprises a net negatively charged filter onto which bacteria are adsorbed and concentrated, an acid diluent which alters the electrostatic charge of the bacteria, thus enabling adsorption of the bacteria to the filter, a bacterial stain operative at about pH 8 to 12, and a washing reagent which enhances the diffusion of free dye away from the immobilized, stained bacterial. The...

대표
청구항

A process for concentrating immobilizing and staining bacteria contained within a fluid sample which bacteria normally possesses a net negative charge, comprising: (a) diluting a sample fluid with an inorganic acid having a pH in the range of from 1 to 3 to convert said negatively charged bacteria to a net positive charge; (b) electrostatically adsorbing said converted net positively charged bacteria in a fluid sample to a designated portion of a net negatively charged filter having a pore size exceeding the size of said bacteria for electrostatic adsorp...

이 특허를 인용한 특허 피인용횟수: 22

  1. Liav Avraham ; Hansjergen Joyce Anne ; Shimasaki Craig David. 4,7-dialkoxy N-acetylneuraminic acid derivatives and methods for detection of influenza type A and B viruses in clinic. USP1998025719020.
  2. Gerd Egger AT. Apparatus for measuring a migration ability of ameboidally mobile cells. USP2002026350610.
  3. Gibbs,Walden Lewis; Gibbs,Jeffrey Dwight. Automated staining and decolorization of biological material. USP2008117452506.
  4. Walden Lewis Gibbs ; Jeffrey Dwight Gibbs. Automated staining and decolorization of biological material. USP2002106468764.
  5. Takagi, Seiichi; Ohta, Tetsuo. Concentration method of fine particle dispersion and concentration apparatus of fine particle dispersion. USP2010117833399.
  6. Peltier, Eric. Device for depositing cells on an analytical plate. USP2003096627158.
  7. Buechler, Kenneth F.; Batt, Richard Roger. Devices for incorporating filters for filtering fluid samples. USP2009097588730.
  8. Putnam, David L.; Putnam, Jason A.; Hubbard, Todd W.. Filtered assay device and method. USP2015038968681.
  9. Putnam, David L.; Putnam, Jason A.; Hubbard, Todd W.; van Zee, James A.. Integrated filtration bioanalyzer. USP2015069068976.
  10. Reinhartz, Avraham; Alajem, Sara. Liquid-transfer device particularly useful as a capturing device in a biological assay process. USP2018049952211.
  11. Robertson, Larry Don; Garrison, Robert Allen. MBI bioaerosol vortex cassette. USP2003106632271.
  12. Robertson, Larry Don; Garrison, Robert Allen. MBI vortex bioaerosol cassette insert. USP2003026517593.
  13. Desmond, Sean M.; Bryning, Zbigniew T.; Shigeura, John; Lim, Gary; Fawcett, Adrian; Freudenthal, Jacob K.; Bordenkircher, Gary. Microfluidic size-exclusion devices, systems, and methods. USP2014018623296.
  14. Desmond,Sean M.; Bryning,Zbigniew T.; Shigeura,John; Lim,Gary; Fawcett,Adrian; Freudenthal,Jacob K.; Bordenkircher,Gary. Microfluidic size-exclusion devices, systems, and methods. USP2007057214348.
  15. West, Jerry; Thomas, Randy; Gibbs, Jeff; Carter, Lee. Multiple slide processing apparatus. USP2015079091621.
  16. West, Jerry; Thomas, Randy; Gibbs, Jeff; Carter, Lee. Multiple slide processing apparatus. USP2016099435720.
  17. Alajem, Sara; Reinhartz, Avraham. Multistep reaction lateral flow capillary. USP2013088507260.
  18. Alajem, Sara; Reinhartz, Avraham. Multistep reaction lateral flow capillary device. USP2015089101927.
  19. Gin, Karina Yew-Hoong; Garrett, Trevor R.. Online contaminant detection and removal system. USP2013068454895.
  20. Gargir, Ari; Semenov, Eugene; Reinhartz, Avraham; Alajem, Sara. Sequential lateral flow capillary device for analyte determination. USP2017089726581.
  21. Om Srivastava CA; Geeta Srivastava CA; Minghui Du CA; Ole Hingsgaul CA; David R. Bundle CA. Syntheses of 4-alkyl chromogenic glycosides and 7-alkyl chromogenic glycosides of N-acetylneuraminic acids. USP2002076420552.
  22. Srivastava Om,CAX ; Srivastava Geeta,CAX ; Du Minghui,CAX ; Hindsgaul Ole,CAX ; Bundle David R.,CAX ; Liav Avraham. Synthesis of 4,7-dialkyl chromogenic glycosides of N-acetylneuraminic acids. USP2001106303764.