초록 ▼

A reaction kit is provided with a reaction zone of suitable cross-sectional area to aspirate a sample by capillarity and a transparent plate with a flat surface in at least part of the reaction zone, and a supporting base to incline the reaction zone at a specified angle. The reaction zone is normal

A reaction kit is provided with a reaction zone of suitable cross-sectional area to aspirate a sample by capillarity and a transparent plate with a flat surface in at least part of the reaction zone, and a supporting base to incline the reaction zone at a specified angle. The reaction zone is normally a space formed by two opposite transparent plates and spacers inserted between these two transparent plates. A supporting base may be molded separately, the transparent plates and spacers being set on the base at the time of measurement, or alternatively the base may be molded integrally with at least one of the plates defining the reaction zone. Further, substances with specificity such as antigens or antibodies may be coated on a surface in the reaction zone. The occurrence or non-occurrence of an agglutination reaction may be determined by introducing a particle suspension containing particles with specific bonding properties and a substance with specific bonding properties into the reaction zone by means of capillarity, leaving the apparatus for a predetermined time to carry out the reaction, and thereafter observing the particle distribution pattern.

대표청구항 ▼

A reaction kit for observation and detection of a particle pattern due to an agglutination reaction, comprising: reaction zone defining means for receiving a sample, said reaction zone defining means comprising: two plates arranged opposed to each other, at least one of said plates being transparent

A reaction kit for observation and detection of a particle pattern due to an agglutination reaction, comprising: reaction zone defining means for receiving a sample, said reaction zone defining means comprising: two plates arranged opposed to each other, at least one of said plates being transparent for visual detection therethrough of a particle pattern formed in said reaction zone; and spacers inserted between said two plates so as to maintain said two plates in spaced apart relation to each other, said reaction zone being defined in a space between said two plates and between said spacers; and a supporting base for supporting said reaction zone defining means, and including means for inclining said reaction zone defining means at a specified angle to thereby incline said reaction zone, such that one of said spacers forms a bottom portion of the inclined reaction zone; a part of the space defined between said two plates and between said spacers serving as an opening through which a sample is introduced into said reaction zone, and wherein said opening is located in a position different from said spacer forming the bottom portion of the inclined reaction zone; wherein a sample is aspirated into said reaction zone when the sample is applied to said reaction zone defining means. A method of determining a degree of particle agglutination by an agglutination reaction having the reaction kit according to claim 1, the method comprising: preparing a particle suspension containing particles with specific bonding properties and a substance with specific bonding affinity to the particles; introducing the particle suspension into a reaction zone of said reaction kit according to claim 1; setting said reaction zone defining means on said supporting base, and leaving said reaction zone defining means, with the particle suspension therein, on said supporting base for a predetermined time; and observing the formation and degree of spread of a particle distribution pattern formed in a lower part of said reaction zone defined by said reaction zone defining means.