초록 ▼

The invention relates to a method of detecting gram-negative bacterial endotoxin using antibody capture combined with amoebocyte lysate chromogenic detection. The method is highly sensitive and rapid and may be used for detection of specific endotoxin. In a particular application, picogram levels of

The invention relates to a method of detecting gram-negative bacterial endotoxin using antibody capture combined with amoebocyte lysate chromogenic detection. The method is highly sensitive and rapid and may be used for detection of specific endotoxin. In a particular application, picogram levels of Haemophilus influenzae are detected in plasma taken from previously infected mammals.

대표청구항 ▼

A method of detecting bacterial endotoxin in a biological fluid, comprising the steps of: contacting a sample of biological fluid with at least one matrix-bound antibody which specifically binds an epitope of bacterial endotoxin to form a matrix-bound bacterial endotoxin; washing the matrix-bound ba

A method of detecting bacterial endotoxin in a biological fluid, comprising the steps of: contacting a sample of biological fluid with at least one matrix-bound antibody which specifically binds an epitope of bacterial endotoxin to form a matrix-bound bacterial endotoxin; washing the matrix-bound bacterial endotoxin; incubating the matrix-bound bacterial endotoxin with an amoebocyte lysate to activate a protease system of the lysate; adding, to the lysate, a substrate of the protease system; and measuring amounts of a product formed from action of said protease system on the added substrate, said amounts being proportional to a level of bacterial endotoxin in the sample.