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Quaternary amine surfactants and methods of using same in isolation of nucleic acids 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C07H-021/00
출원번호 US-0013419 (1993-02-01)
발명자 / 주소
  • Macfarlane Donald E. (Iowa City IA)
출원인 / 주소
  • University of Iowa Research Foundation (Iowa City IA 02)
인용정보 피인용 횟수 : 42  인용 특허 : 0

초록

The present invention discloses a novel method for isolating nucleic acids from biological samples, most particularly blood, using selected quaternary amine surfactants. The nucleic acids are isolated quickly and in sufficient quantity and quality to permit analysis by methods including reverse tran

대표청구항

A method for isolating nucleic acids from a biological sample comprising the step of contacting the sample with an aqueous quaternary amine surfactant, wherein said quaternary amine has the formula [Figure] wherein R1 through R4 are independently selected from an alkyl chain containing from the 1 to

이 특허를 인용한 특허 (42)

  1. Chen,Xu Qi; Stroun,Maurice; Anker,Philippe, Cancer diagnosis method.
  2. Kopreski, Michael S., Comparative analysis of extracellular RNA species.
  3. Kopreski, Michael S., Comparative analysis of extracellular RNA species.
  4. Becker Michael McClellan, Compositions and methods for detecting and quantifying biological samples.
  5. Becker Michael McClellan, Compositions for detecting and quantifying biological samples.
  6. Holl채nder,Vera; Wyrich,Ralf; Oelm체ller,Uwe, Compositions for isolating and/or stabilising nucleic acids in biological material.
  7. Holländer, Vera; Wyrich, Ralf; Oelmüller, Uwe, Compositions for the isolation and/or stabilization of nucleic acids in biological materials.
  8. Greenfield, Lawrence; Montesclaros, Luz, Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases.
  9. Greenfield,I. Lawrence, Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases.
  10. Greenfield,Lawrence; Montesclaros,Luz, Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases.
  11. Schneider Claudio (Udine ITX), Extraction of genomic DNA from blood using cationic detergents.
  12. Augello, Frank A.; Rainen, Lynne; Walenciak, Matthew; Oelmüller, Uwe; Wyrich, Ralf; Bastian, Helge, Method and device for collecting and stabilizing a biological sample.
  13. Augello, Frank A.; Rainen, Lynne; Walenciak, Matthew; Oelmuller, Uwe; Wyrich, Ralf; Bastian, Helge, Method and device for collecting and stabilizing a biological sample.
  14. Augello, Frank A.; Rainen, Lynne; Walenciak, Matthew; Oelmuller, Uwe; Wyrich, Ralf; Bastian, Helge, Method and device for collecting and stabilizing a biological sample.
  15. Kopreski, Michael S., Method enabling the use of extracellular ribonucleic acid (RNA) extracted from plasma or serum to detect, monitor or evaluate cancer or premalignant conditions.
  16. Kopreski Michael S., Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer.
  17. Kopreski, Michael, Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer.
  18. Kopreski, Michael, Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer.
  19. Kopreski, Michael, Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer.
  20. Kopreski, Michael S., Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer.
  21. Kopreski, Michael S., Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer.
  22. Kopreski, Michael S., Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer.
  23. Kopreski, Michael S., Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer.
  24. Kopreski, Michael S., Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer.
  25. Singer, Thorsten, Method for cleaning and isolating nucleic acid using ammonium or phosphonium cationic detergents.
  26. Stoy Patrick, Method for creation of biomimetic surfaces.
  27. Stead, Mark B.; Kushner, Sidney R., Method for isolating total RNA from cells.
  28. Korfhage, Christian; Wilmer, Friederike; Loffert, Dirk; Himmelreich, Ralf; Fritz, Claudia; Rieske, Kathleen, Method for the treatment of a sample containing biomolecules.
  29. Korfhage, Christian; Wilmer, Friederike; Löffert, Dirk; Himmelreich, Ralf; Fritz, Claudia; Rieske, Kathleen, Method for the treatment of a sample containing biomolecules.
  30. Korfhage, Christian; Wilmer, Friederike; Löffert, Dirk; Himmelreich, Ralf; Fritz, Claudia; Rieske, Kathleen, Method for the treatment of a sample containing biomolecules.
  31. Erbacher, Christoph; Bastian, Helge; Wyrich, Ralf; Oelmüller, Uwe; Manz, Thomas, Method of stabilizing and/or isolating nucleic acids.
  32. Kopreski, Michael S., Methods for detecting and monitoring cancer using extracellular RNA.
  33. Gundling, Gerard, Nucleic acid isolation method and kit.
  34. Coffman Jonathan L., One step device and process for concentration and purification of biological molecules.
  35. Macfarlane Donald E., Quaternary amine surfactant and methods of using same in isolation of RNA.
  36. Soon-Jong Hahn KR; Jeong-Joo Shin KR; Jae-Min Ha KR; Chang-Mook Cho KR; Ki-Man Park KR; Kwang-Hee Yoo KR; Dong-Jin Choi KR; Jun-Weon Park KR, Quaternary ammonium phosphate compound and method of preparing same.
  37. Mehra Manmohan, RNA separation.
  38. Baker, Tony, Removal of molecular assay interferences.
  39. Goldsborough, Andres Simon; Bates, Malcolm Robert, Sample fixation and stabilisation.
  40. Oelm?ller, Uwe; Wille, Tanja, Use of compositions consisting of cationic compounds and proton donors for stabilizing and/or isolating nucleic acids in or from micro-organisms such as prokaryots, fungi, protozoa or algae.
  41. Helftenbein, Elke, Vessel for blood sampling.
  42. Ekeze Tobias E. (Brockport NY) Kerschner JoAnne Hansen (Rochester NY), Whole blood sample preparation for polymerase chain reaction using ammonium chloride and a carboxylic acid or metal carb.
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