로그인시 본인확인과정이 필요할 수 있습니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
DataON 바로가기
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Edison 바로가기
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Kafe 바로가기
| 국가/구분 | United States(US) Patent 등록 |
|---|---|
| 국제특허분류(IPC7판) |
|
| 출원번호 | US-0796947 (1991-11-22) |
| 발명자 / 주소 |
|
| 출원인 / 주소 |
|
| 인용정보 | 피인용 횟수 : 275 인용 특허 : 0 |
A method and device for measuring the binding affinity of a receptor to a ligand. According to one aspect of the invention, arrays of polymers are synthesized or immobilized on a substrate (212). The array of polymers is exposed to a fluorescently-labelled receptor in solutions of varying concentrat
A method and device for measuring the binding affinity of a receptor to a ligand. According to one aspect of the invention, arrays of polymers are synthesized or immobilized on a substrate (212). The array of polymers is exposed to a fluorescently-labelled receptor in solutions of varying concentration. The fluorescence intensity of the labelled receptor is measured by way of, e.g., a photon counter using a confocal microscope (316). Binding affinity is determined through analysis of the relationship between fluorescence intensity and the solution concentration of the receptor. On-rates are measured as a kinetic increase in surface fluorescence intensity, and the on-rate constant rate extracted from fits to the data.
A method of determining the presence and strength of binding affinity, Kd, of a receptor, R, for a selected polymer attached at a selected region on a surface of a substrate comprising an array of polymers attached at a plurality of selected regions on the surface of the substrate, comprising the st
A method of determining the presence and strength of binding affinity, Kd, of a receptor, R, for a selected polymer attached at a selected region on a surface of a substrate comprising an array of polymers attached at a plurality of selected regions on the surface of the substrate, comprising the steps of: (a) exposing the polymers to a first concentration solution of said receptor, said receptor being labeled with a fluorescent marker; (b) irradiating the polymers with a light source; (c) measuring fluorescence intensity of each selected region with a confocal microscope at a time sufficient for the receptor to reach specific binding equilibrium with the selected polymer; (d) repeating steps (a)-(c) with a second concentration solution of said receptor, said receptor being labeled with the fluorescent marker, wherein the measuring step occurs while at least one of said solutions is in contact with the substrate; (e) calculating Kd from the fluorescence intensities and the receptor concentrations for the selected polymer by solving an equation, [Figure] wherein I=fluorescence intensity, [R]=receptor concentration, Imax=a saturation fluorescence intensity correction factor, to determine the presence and strength of binding of the receptor for the selected polymer.
Copyright KISTI. All Rights Reserved.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.