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특허 상세정보

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내보내기 페이스북 공유 카카오톡 공유

In situ PCR amplification system

특허상세정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) C12M-001/40   
미국특허분류(USC) 435/288 ; 435/290 ; 435/316 ; 435/809
출원번호 US-0017721 (1993-02-16)
발명자 / 주소
출원인 / 주소
인용정보 피인용 횟수 : 59  인용 특허 : 0
초록
▼

A complete in situ PCR system for amplification of nucleic acids contained in a prepared cell or tissue sample. The containment system for the PCR sample comprises a glass microscope slide, a specimen sample containing the target nucleic acid sequence mounted on the slide, a flexible plastic cover over the sample, and a retaining assembly fastened to the slide and to the cover to retain and seal a reaction mixture against the sample during thermal cycling. The retaining assembly includes a rigid ring on a rim portion of the cover, a cross beam having spa...

대표
청구항

A retaining assembly for retaining a fluid against a glass slide comprising a resilient and compliant cover member, an annular seal member around at least a portion of said cover member and at least one clip means for removably retaining said cover and seal members against said slide.

이 특허를 인용한 특허 피인용횟수: 59

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  18. Adey,Nils B.. Laminated microarray interface device. USP2007067235400.
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  45. Wittwer Carl T. ; Ririe Kirk M. ; Rasmussen Randy P.. PCR method for nucleic acid quantification utilizing second or third order rate constants. USP2001056232079.
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  59. Oldham, Mark F.; Fawcett, Adrian. Vacuum assist for a microplate. USP2015129213042.

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