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In situ PCR amplification system 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12M-001/40
출원번호 US-0017721 (1993-02-16)
발명자 / 주소
  • Atwood John G. (West Redding CT) Haff Lawrence A. (Wilton CT)
출원인 / 주소
  • The Perkin-Elmer Corporation (Norwalk CT 02)
인용정보 피인용 횟수 : 59  인용 특허 : 0

초록 ▼

A complete in situ PCR system for amplification of nucleic acids contained in a prepared cell or tissue sample. The containment system for the PCR sample comprises a glass microscope slide, a specimen sample containing the target nucleic acid sequence mounted on the slide, a flexible plastic cover o

대표청구항

A retaining assembly for retaining a fluid against a glass slide comprising a resilient and compliant cover member, an annular seal member around at least a portion of said cover member and at least one clip means for removably retaining said cover and seal members against said slide.

이 특허를 인용한 특허 (59)

  1. Walker Francis J. ; Johnson Theodore D., Anti-bleed coating for silicone automobile gaskets.
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  2. Chu, Wei Sing, Apparatus and methods for efficient processing of biological samples on slides.
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  3. Chu, Wei-Sing, Apparatus and methods for efficient processing of biological samples on slides.
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  4. Chu Wei-Sing, Apparatus for efficient processing of tissue samples on slides.
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  5. Andrevski Zygmunt M. ; Roach William Ronald ; Southgate Peter David ; Zanzucchi Peter John, Assay system and method for conducting assays.
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  6. Belz, Renato; Carsten, Haack, Chip based diagnostic device.
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  7. Wittwer, Carl T.; Ririe, Kirk M., Container for carrying out and monitoring biological processes.
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  8. Wittwer,Carl T.; Ririe,Kirk M., Container for carrying out and monitoring biological processes.
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  9. Domanik Richard A. ; Dadeshidze Vladimir ; Olsson Lars J., Cytological specimen analysis system with slide mapping and generation of viewing path information.
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  10. Villa,Pascal; Delaage,Michel; Williamson,Toni, Device for sequential observation of samples and methods using same.
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  11. Lorincz Andrew E., Flexible microscope slide.
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  12. Wittwer Carl T., Fluorescent donor-acceptor pair with low spectral overlap.
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  13. Kubu, Christopher J.; Muller-Greven, Jeannine C.; Moffett, Robert B., Hot start nucleic acid amplification.
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  14. Kubu, Christopher James; Muller-Greven, Jeannine Catherine; Moffett, Robert Bruce, Hot start nucleic acid amplification.
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  15. Nagle, Robert; Dubrow, Robert S., Integrated devices and method of use for performing temperature controlled reactions and analyses.
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  16. Nagle Robert ; Dubrow Robert S., Integrated devices and systems for performing temperature controlled reactions and analyses.
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  17. Hsieh,Huangpin Ben; Kneissl,Michael A., LED or laser enabled real-time PCR system and spectrophotometer.
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  18. Adey,Nils B., Laminated microarray interface device.
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  19. Keating Mark T. (Salt Lake City UT) Curran Mark E. (Salt Lake City UT) Wang Qing (Salt Lake City UT), Long QT syndrome genes.
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  20. Mack J. Schermer ; C. Todd Lombardo ; Robert Milkowski ; Donald Regan ; Paul St. Cyr, Method and apparatus for incubation of a liquid reagent and target spots on a microarray substrate.
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  21. Hu Can B. ; Ma Minh T. ; Nguyen Than ; Rhee Richard ; Myers Keith, Method and apparatus for sodding microvessel cells onto a synthetic vascular graft.
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  22. Kram, Brian H., Method and apparatus for treating a biological sample with a liquid reagent.
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  23. McNeely,Michael; Adey,Nils; Spute,Mark; Ayliffe,Edward; Howard,Michael; Beutel,Darin; Jensen,John; Coffin,Stephen; Moyer,Thomas, Method and system for microfluidic interfacing to arrays.
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  24. Ohno, Tsuneya; Matsuhisa, Akio, Method for detecting and identifying microorganism causative of infection.
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  25. Robert Nagle ; Robert S. Dubrow, Methods and integrated devices and systems for performing temperature controlled reactions and analyses.
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  26. Mayrand, Paul E., Methods and reagents for combined PCR amplification.
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  27. Mayrand,Paul E., Methods and reagents for combined PCR amplification.
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  28. Mayrand,Paul E., Methods and reagents for combined PCR amplification and hybridization probing.
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  29. Paul E. Mayrand, Methods and reagents for combined PCR amplification and hybridization probing.
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  30. Lorincz Andrew Endre, Microscope slide.
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  31. Fisch Harry, Microscope slide assembly.
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  32. Fisch Harry, Microscope slide assembly.
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  33. Fisch Harry, Microscope slide assembly.
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  34. Fisch Harry, Microscope slide assembly.
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  35. Lorincz, Andrew E., Microscope slide having culture media and method for use.
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  36. Maerzhaeuser, Guenter; Scherer, Sebastian, Microscope table.
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  37. Maerzhaeuser, Guenter; Scherer, Sebastian, Microscope table.
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  38. Wittwer Carl T. ; Ririe Kirk M. ; Rasmussen Randy P., Monitoring amplification of DNA during PCR.
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  39. Wittwer, Carl T.; Ririe, Kirk M.; Rasmussen, Randy P., Monitoring hybridization during PCR using SYBR® Green I.
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  40. Yamamoto Soji,JPX ; Endo Itaru,JPX ; Kaneko Yasushi,JPX, Movable objective lens type optical microscope having stationary stage and specimen moving stage.
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  41. Hall, John P.; Muser, Andrew P.; Whitley, Kenneth W., Multi-well plate cover and assembly adapted for mechanical manipulation.
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  42. Domanik Richard A. ; Gruber Dennis W. ; Gombrich Peter P. ; Mayer William J., Multifunctional control unit for a microscope.
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  43. Kawaguchi, Masahiro, Nucleic-acid probe substrate, system for temperature control of the substrate, and gene detection method making use of the same.
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  44. Swerdlow Harold P. ; Wittwer Carl T., On-line DNA analysis system with rapid thermal cycling.
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  45. Wittwer Carl T. ; Ririe Kirk M. ; Rasmussen Randy P., PCR method for nucleic acid quantification utilizing second or third order rate constants.
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  46. Green, Larry R., Portable biosensor apparatus with controlled flow.
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  47. Nishimura Naoyuki,JPX ; Nakayama Tomoko,JPX, Process and reagent for amplifying nucleic acid sequences.
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  48. Lee, Martin Alan; Squirrell, David James; Jones, Ross Peter; Williamson, Roger James; Gregory, George Richard; Gutsell, Graham, Reaction vessel comprising conductive layer and inner non-metallic layer.
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  49. Freeman Thomas Charles,GBX, Sample processing device with a chamber forming member.
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  50. Thomas Charles Freeman GB, Sample processing device with a chamber forming member.
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  51. Lee, Martin Alan; Squirrell, David James; Withers, Michael John; Beckett, Trevor John; Silk, Christopher John, Sample processor.
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  52. Sandell, Donald R., Slip cover for heated platen assembly.
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  53. Eichele, Gregor; Herzig, Uwe, Specimen chamber for the liquid treatment of biological specimens.
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  54. Domanik Richard A ; Gombrich Peter G. ; Mayer William J., Specimen management system.
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  55. Wittwer,Carl T.; Ririe,Kirk M.; Rasmussen,Randy P.; Hillyard,David R., System and method for fluorescence monitoring.
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  56. Wittwer, Carl T.; Ririe, Kirk M., System for fluorescence monitoring.
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  57. Domanik Richard A. ; Gruber Dennis W. ; Mayer William J., System for simplifying the implementation of specified functions.
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  58. Christner James E. ; Price Kenneth W., Test strip incubation device.
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  59. Oldham, Mark F.; Fawcett, Adrian, Vacuum assist for a microplate.
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