IPC분류정보
| 국가/구분 |
United States(US) Patent
등록
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| 국제특허분류(IPC7판) |
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| 출원번호 |
US-0156519
(1993-11-23)
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발명자
/ 주소 |
- Ness Jeffrey V. (Bothell WA) Cimler B. Melina (Portland OR) Meyer
- Jr. Rich B. (Woodinville WA) Vermeulen Nicolaas M. J. (Woodinville WA)
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| 출원인 / 주소 |
- MicroProbe Corporation (Bothell WA 02)
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| 인용정보 |
피인용 횟수 :
32 인용 특허 :
0 |
초록
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This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction so
This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase. Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction. The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.
대표청구항
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An extraction composition for the isolation of released nucleic acids comprising: at least one organic compound which is a liquid at room temperature and which is selected from the group of compounds having the formula C6H5CH2OH or: [Figure] where: R1 is －CH2OH, R2 is a member selected from the grou
An extraction composition for the isolation of released nucleic acids comprising: at least one organic compound which is a liquid at room temperature and which is selected from the group of compounds having the formula C6H5CH2OH or: [Figure] where: R1 is －CH2OH, R2 is a member selected from the group consisting of －H, －F, －C, －Br, －I, －OH, －S(CH2)rCH3 in which r is 0 to 3, －O(CH2)qCH3 in which q is 0 to 6, －COOR6 in which R6 is －(CH2)sCH3 in which s is 0 to 3, －C6H5, －CH2C6H5 , －OC6H5, －OCH2C6H5, －CH2OH, －CF3, and －(CH2)tCH3 in which t is 0 to 6 and R3 and R4 are both －H; or R1 is －CH2OH, R2 and R3 are the same member selected from the group consisting of －H, －F, －Cl, －Br, －I, －OH, －(CH2)xCH3 in which x is 0 to 3, －O(CH2)vCH3 in which v is 0 to 3 and R4 is －H; or R1 is －CH2OH, R2, R3 and R4 are the same member selected from the group consisting of －H, －F, －Cl, －Br, －I, －(CH2)yCH3 in which y is 0 to 3, －O(－CH2)wCH3 in which w is 0 to 3; or R1 is －CH2OH, R2 is －C6H5, －OCH2C6H5, or －CH2C6H5, R3 is －CH3, －OH, －Cl, －Br, －F, －I, －H, －OCH3 or －OCH2CH3 and R4 is －H; and a clay or a silicate or an admixture of said clay and said silicate; wherein said clay or said silicate or said admixture is 0.1% to about 5% by weight of said composition.
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