Spatially-addressable immobilization of anti-ligands on surfaces
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-031/415
C07D-491/048
출원번호
US-0053124
(1993-04-23)
발명자
/ 주소
Barrett Ronald W. (Sunnyvale CA) Pirrung Michael C. (Durham NC) Stryer Lubert (Stanford CA) Holmes Christopher P. (Sunnyvale CA) Sundberg Steven A. (San Francisco CA)
출원인 / 주소
Affymax Technologies N.V. (03)
인용정보
피인용 횟수 :
201인용 특허 :
0
초록▼
Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise
Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise protecting groups capable of being removed upon application of a suitable energy source. Spatially addressed irradiation of predefined regions on the surface permits immobilization of anti-ligands at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allows formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix. A preferred embodiment of the invention involves attaching photoactivatable biotin derivatives to a surface. Photolytic activation of the biotin derivatives forms biotin analogs having strong binding affinity for avidin. Biotinylated anti-ligands can be immobilized on activated regions of the surface previously treated with avidin.
대표청구항▼
A caged binding member compound having the formula: [Figure] wherein X and Z are selected from the group consisting of (a) hydrogen, (b) oxycarbonyls of lower alkyl, aryl, and benzyl groups, (c) a nitroveratryloxycarbonyl group, (d) a nitropiperonyloxycarbonyl group where Z is hydrogen and R is p-ni
A caged binding member compound having the formula: [Figure] wherein X and Z are selected from the group consisting of (a) hydrogen, (b) oxycarbonyls of lower alkyl, aryl, and benzyl groups, (c) a nitroveratryloxycarbonyl group, (d) a nitropiperonyloxycarbonyl group where Z is hydrogen and R is p-nitrophenyl formate, (e) [Figure] (e) wherein R1 and R2 are hydrogen, lower alkyl, aryl, benzyl, halogen, hydroxyl, alkoxyl, thiol, thioether, amino, nitro, carboxyl, formate, sulfonate, formamido or phosphido groups, (f) [Figure] (f) wherein R1 and R2 are hydrogen, lower alkyl, aryl, benzyl, pyrenyl, halogen, hydroxyl, alkoxyl, thiol, thioether, amino, nitro, carboxyl, formate, formamido or phosphido groups, and R3 and R4 are hydrogen, lower alkyl, aryl, benzyl, halogen, hydroxyl, alkoxyl, thiol, thioether, amino, nitro, carboxyl, formate, formamido or phosphido groups, (g) [Figure] (g) wherein R1 and R2 are hydrogen, lower alkyl, aryl, benzyl, halogen, hydroxyl, alkoxyl, thiol, thioether, amino, nitro, carboxyl, formate, formamido or phosphido groups, and (h) [Figure] (h) wherein R1, R2, R3 and R4 are hydrogen, lower alkyl, aryl, benzyl, halogen, hydroxyl, alkoxyl, thiol, thioether, amino, nitro, carboxyl, formate, formamido or phosphido groups, provided that when X is hydrogen, Z is not hydrogen or methyloxycarbonyl and provided that when Z is hydrogen, X is not hydrogen or methyloxycarbonyl; R is selected from the group consisting of hydrogen, lower alkyl, aryl, carboxylate lower alkyl formate, aryl formate, methyl formate, p-nitrophenyl formate, formamide, N-alkylformamide, N-succinimidyl, hydroxyl, alkoxyl, thiol, thioether, disulfide, hydrazide and amine groups, provided that when X or Z is methyloxycarbonyl, R is not methyl formate; U is O, S or NH; Y is selected from the group consisting of sulfur, oxygen, methylene, carbonyl, sulfinyl and sulfonyl groups, or Y represents two hydrogens attached to the respective carbons; n=0-7; or acid addition salts of the compound.
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