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Chromosomal expression of non-bacterial genes in bacterial cells 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12N-015/64
  • C12N-015/66
출원번호 US-0170588 (1993-12-21)
발명자 / 주소
  • Mascarenhas Desmond (San Rafael CA) Olson Pamela S. (Cupertino CA)
출원인 / 주소
  • Celtrix Pharmaceuticals, Inc. (Santa Clara CA 02)
인용정보 피인용 횟수 : 40  인용 특허 : 0

초록 ▼

The present invention provides compositions and methods for inserting a copy of a heterologous gene into the chromosome of a host cell such as E. coli through the use of a chromosomal transfer DNA, a circular, non-self-replicating DNA carrying a site-specific recombination site such as lambda att P.

대표청구항 ▼

A method for introducing a non-bacterial gene of interest into a chromosome of a host cell comprising the steps of: ligating a restriction fragment from a first plasmid vector with a restriction fragment from a second plasmid vector, wherein the first plasmid vector comprises a first origin of repli

이 특허를 인용한 특허 (40)

  1. Kourtz, Lauralynn; Peoples, Oliver P.; Snell, Kristi D., Chemically Inducible Expression of Biosynthetic Pathways.
    상세보기
  2. Kourtz, Lauralynn; Peoples, Oliver P.; Snell, Kristi D., Chemically inducible expression of biosynthetic pathways.
    상세보기
  3. Olson Pamela S. ; Mascarenhas Desmond, Chromosomal expression of heterologous genes in bacterial cells.
    상세보기
  4. Brasch, Michael; Cheo, David; Hartley, James; Temple, Gary, Compositions for use in recombinational cloning of nucelic acids.
    상세보기
  5. Hartley, James L.; Brasch, Michael A.; Temple, Gary F.; Cheo, David, Compositions for use in recombinational cloning of nucleic acids.
    상세보기
  6. Hartley, James; Brasch, Michael; Temple, Gary; Cheo, David, Compositions for use in recombinational cloning of nucleic acids.
    상세보기
  7. Gerard, Gary F.; Flynn, Elizabeth; Hu, A-Li W., Enhanced in vitro recombinational cloning of using ribosomal proteins.
    상세보기
  8. Farmer, William R.; Bickmeier, Jeff; Lu, Chenfeng; Chang, Dong-Eun; Skraly, Frank; Ramseier, Thomas Martin, Green process and compositions for producing poly(5HV) and 5 carbon chemicals.
    상세보기
  9. Godiska, Ronald; Mead, David A.; Ravin, Nikolai V., Linear vectors, host cells and cloning methods.
    상세보기
  10. Godiska, Ronald; Mead, David A.; Ravin, Nikolai V., Linear vectors, host cells and cloning methods.
    상세보기
  11. Calos, Michele P., Methods and compositions for genomic modification.
    상세보기
  12. Calos, Michele P., Methods and compositions for genomic modification.
    상세보기
  13. Calos,Michele Pamela, Methods and compositions for genomic modification.
    상세보기
  14. Chesnut, Jonathan D.; Carrino, John; Leong, Louis; Madden, Knut; Gleeson, Martin; Fan, James; Brasch, Michael; Cheo, David; Hartley, James L.; Byrd, Devon R.; Temple, Gray F., Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites.
    상세보기
  15. Chesnut, Jonathan; Carrino, John; Leong, Louis; Madden, Knut; Gleeson, Martin; Fan, James; Brasch, Michael; Cheo, David; Hartley, James; Byrd, Devon; Temple, Gary, Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites.
    상세보기
  16. Chesnut,Jonathan D.; Carrino,John; Leong,Louis; Madden,Knut; Gleeson,Martin A. G.; Fan,James; Brasch,Michael A.; Cheo,David; Hartley,James L.; Byrd,Devon R. N.; Temple,Gary F., Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites.
    상세보기
  17. Chestnut,Jonathan D.; Carrino,John; Leong,Louis; Madden,Knut; Gleeson,Martin; Fan,James; Brasch,Michael A.; Cheo,David; Hartley,James L.; Byrd,Devon R.N.; Temple,Gary F., Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites.
    상세보기
  18. Chesnut, Jonathan; Carrino, John; Leong, Louis; Madden, Knut; Gleeson, Martin; Fan, James; Brasch, Michael; Cheo, David; Hartley, James; Byrd, Devon; Temple, Gary, Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites.
    상세보기
  19. Helliwell, Christopher A.; Wesley, Susan V.; Waterhouse, Peter M., Methods and means for producing efficient silencing construct using recombinational cloning.
    상세보기
  20. Helliwell, Christopher A.; Wesley, Susan V.; Waterhouse, Peter M., Methods and means for producing efficient silencing construct using recombinational cloning.
    상세보기
  21. Chesnut, Jon; Leong, Louis, Nucleic acid molecules containing recombination sites and methods of using the same.
    상세보기
  22. Chesnut, Jonathan; Leong, Louis, Nucleic acid molecules containing recombination sites and methods of using the same.
    상세보기
  23. Elledge, Stephen J.; Liu, Qinghua, Rapid subcloning using site-specific recombination.
    상세보기
  24. Sumner,Emma Jayne; Hodgson,Ian, Recombinant IGF expression systems.
    상세보기
  25. Hartley James L. ; Brasch Michael A., Recombination cloning using engineered recombination sites.
    상세보기
  26. Hartley James L. ; Brasch Michael A., Recombinational cloning using engineered recombination sites.
    상세보기
  27. Hartley James L. ; Brasch Michael A., Recombinational cloning using engineered recombination sites.
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  28. Hartley, James L.; Brasch, Michael A., Recombinational cloning using engineered recombination sites.
    상세보기
  29. Hartley,James L.; Brasch,Michael A., Recombinational cloning using engineered recombination sites.
    상세보기
  30. Hartley,James L.; Brasch,Michael A., Recombinational cloning using engineered recombination sites.
    상세보기
  31. Hartley,James L.; Brasch,Michael A., Recombinational cloning using engineered recombination sites.
    상세보기
  32. Hartley James L. ; Brasch Michael A. ; Temple Gary F. ; Fox Donna K., Recombinational cloning using nucleic acids having recombination sites.
    상세보기
  33. Hartley,James L.; Brasch,Michael A.; Temple,Gary F.; Fox,Donna K., Recombinational cloning using nucleic acids having recombination sites.
    상세보기
  34. Huisman, Gjalt W.; Peoples, Oliver P.; Skraly, Frank A., Transgenic microbial polyhydroxyalkanoate producers.
    상세보기
  35. Huisman, Gjalt W.; Peoples, Oliver P.; Skraly, Frank A., Transgenic microbial polyhydroxyalkanoate producers.
    상세보기
  36. Madison,Lara; Huisman,Gjalt W.; Peoples,Oliver P., Transgenic systems for the manufacture of poly (3-hydroxy-butyrate-co-3-hydroxyhexanoate).
    상세보기
  37. Madison, Lara; Huisman, Gjalt W.; Peoples, Oliver P., Transgenic systems for the manufacture of poly(2-hydroxy-butyrate-co-3-hydroxyhexanoate).
    상세보기
  38. Madison,Lara; Huisman,Gjalt W.; Peoples,Oliver P., Transgenic systems for the manufacture of poly(2-hydroxy-butyrate-co-3-hydroxyhexanoate).
    상세보기
  39. Cheo,David; Brasch,Michael A.; Temple,Gary F.; Hartley,James L.; Byrd,Devon R. N., Use of multiple recombination sites with unique specificity in recombinational cloning.
    상세보기
  40. Cheo,David; Brasch,Michael A.; Temple,Gary F.; Hartley,James L.; Byrd,Devon R. N., Use of multiple recombination sites with unique specificity in recombinational cloning.
    상세보기

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