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| 국가/구분 | United States(US) Patent 등록 |
|---|---|
| 국제특허분류(IPC7판) |
|
| 출원번호 | US-0308199 (1994-09-19) |
| 발명자 / 주소 |
|
| 출원인 / 주소 |
|
| 인용정보 | 피인용 횟수 : 781 인용 특허 : 0 |
Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction. The devices comprise a substrate microfabricated to define a sample inlet port and a mesoscale flow system, which extends from the inlet port. The mesoscale flow syst
Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction. The devices comprise a substrate microfabricated to define a sample inlet port and a mesoscale flow system, which extends from the inlet port. The mesoscale flow system includes a polynucleotide polymerization reaction chamber in fluid communication with the inlet port which is provided with reagents required for polymerization and amplification of a preselected polynucleotide. In one embodiment the devices may be utilized to implement a polymerase chain reaction (PCR) in the reaction chamber (PCR chamber). The PCR chamber is provided with the sample polynucleotide, polymerase, nucleoside triphosphates, primers and other reagents required for the polymerase chain reaction, and the device is provided with means for thermally controlling the temperature of the contents of the reaction chamber at a temperature controlled to dehybridize double stranded polynucleotide, to anneal the primers, and to polymerize and amplify the polynucleotide.
A device for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction, the device comprising: a solid substrate which is microfabricated to define: a sample inlet port; a mesoscale flow system comprising: a sample flow channel extending from said inle
A device for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction, the device comprising: a solid substrate which is microfabricated to define: a sample inlet port; a mesoscale flow system comprising: a sample flow channel extending from said inlet port; and a polynucleotide amplification reaction chamber, in fluid communication with said flow channel; said sample flow channel and said reaction chamber having at least one cross-sectional dimension of width or depth which is about 0.1 to 500 m
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