초록 ▼

A connection type fluid transfer and treatment system apparatus and method for efficiently and continuously executing transfer and treatment of small or micro amounts of sample solutions without substantial transfer loss, which includes a first microsolution reaction microtube having one open end an

A connection type fluid transfer and treatment system apparatus and method for efficiently and continuously executing transfer and treatment of small or micro amounts of sample solutions without substantial transfer loss, which includes a first microsolution reaction microtube having one open end and a second closed end, a second microsolution target microtube having substantially the same shape as the first microtube also having one open end and one closed end, and a connector for connecting together the open ends of the first microtube to the open end of the second tube. The connector includes a foramenous membrane support, which removably receives chemically or biologically treated membranes for applying a predetermined treatment to a solution while passing the sample solution from the first microtube to the second tube. Alternately, a “single use only”connector having disposed therein a membrane pretreated with a quantitative amount of reagent may be used for certain treatment operations. The single use only connector is used once and then discarded. An appropriate color indicator in the connector or membrane would serve to indicate whether the connector had been used. The sample is typically filtered through the membrane by centrifugation. The assembly also includes special adapters for receivingly engaging the dual tube/connector transfer system during centrifugation. The system permits handling microliter quantities of reactive solutions in biochemical analyses, treatments and assays without use of micropipets, without the usual loss of solution. An enzyme microsolution test kit system and method of use comprising one or more micro solution microtubes containing a predetermined quantity of a known, preferably lyophilized, reagent pre-coated on the microtube walls is disclosed.

대표청구항 ▼

A dual invertible microtube and connector assembly for transfer and treatment of micro solutions and centrifugation comprising in operative combination: a) a first source microtube having a first open end and terminating in a second, tapered, permanently closed end, said first microtube adapted to c

A dual invertible microtube and connector assembly for transfer and treatment of micro solutions and centrifugation comprising in operative combination: a) a first source microtube having a first open end and terminating in a second, tapered, permanently closed end, said first microtube adapted to contain a micro solution sample therein for treatment by inversion; b) a second target microtube having substantially the same shape as said first microtube and having a first open end and terminating in a second, tapered, permanently closed end; c) each of said microtubes includes adjacent to its open end: i) threads disposed along a first peripheral wall surface; and ii) a smooth, cylindrical second peripheral wall surface; d) a connector assembly for connecting the open end of said first microtube to the open end of said second microtube, said connector assembly having a first generally cylindrical connector end having a smooth inner peripheral wall surface for slip fit connection about an outer peripheral wall of the open end of either of said first or second microtubes, and a second threaded connector end for threaded engagement with the threaded open end of the other one of said first or second microtubes; e) a generally tubular membrane support having an outer peripheral wall, an inner peripheral wall and a generally flat foraminous membrane support surface disposed within said inner peripheral wall, said inner peripheral wall and said membrane foraminous support defining a central cavity for insertion within the open end of the microtube having said first connector end slip-fitted thereover; f) a single means for securing a membrane to said foraminous surface of said membrane support in a manner to provide a liquid tight seal between the periphery of said membrane support and said membrane; g) said membrane support in combination with said securing means retaining said membrane at a recessed distance within the open end of either of said first or second microtubes for filtering and applying a predetermined treatment to said sample solution while passing said sample solution from said first source microtube into said second target microtube by inversion of said first source microtube containing said sample solution to be filtered over said second target microtube; and h) said dual invertible microtube and connector assembly are adapted for inversion for unvented treatment of said sample solution, and for direct centrifugation of treated samples retained in said microtubes.