초록

Methods useful in purifying nucleic acids from whole cell samples are described. This is accomplished by adding one or both of a non-phenyl group containing non-ionic detergent or a cross-linked, polycarboxylic acid. Kits which can be used in the methods are also described.

대표청구항 ▼

Method for amplifying a nucleic acid molecule of interest, comprising (i) contacting a whole-cell-containing sample with at least one sodium dodecyl sulfate containing lysis buffer to lyse nucleic acid containing cells; (ii) adding to said sample an amount of a water insoluble cross linked polyhydro

Method for amplifying a nucleic acid molecule of interest, comprising (i) contacting a whole-cell-containing sample with at least one sodium dodecyl sulfate containing lysis buffer to lyse nucleic acid containing cells; (ii) adding to said sample an amount of a water insoluble cross linked polyhydroxy polycarboxylic acid having at least two strands of formula [Figure] wherein one carbonyl group of at least one maleoyl moiety thereof in each strand is covalently linked to a -HN[(H)p(CH)2(OH)m]NH-moiety to provide the presence therein of at least one cross linking moiety of the formula: [Figure] wherein R is hydrogen or lower alkylene or lower alkoxy of 1-4 carbon atoms, or phenyl, z is an integer of 1-4, p is 0 or an integer up to z-1, m is 1 or an integer up to z, wherein the ratio of cross-links to poly(alkylene carbonic acid) strands is between about 1 and about 200 to 2 sufficient to sequester any proteins in said sample; (iii) recovering nucleic acids in said sample, wherein said recovering is accomplished without alcohol precipitation; and (iv) adding to said nucleic acids an amplification reagent which specifically amplifies said nucleic acid molecule of interest.