[특허]Method and apparatus for covalent immobilization of charge- conjugated carbohydrate molecules

Method and apparatus for covalent immobilization of charge- conjugated carbohydrate molecules 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	B01D-061/38 C07H-001/06 G01N-027/26
출원번호	US-0157805 (1993-11-24)
발명자 / 주소	Charkoudian John (Carlisle MA) Pluskal Malcolm (Acton MA) Wang David (Lexington MA) Phoebe Charles (Uxbridge MA)
출원인 / 주소	Millipore Corporation (Bedford MA 02)
인용정보	피인용 횟수 : 40 인용 특허 : 0

초록 ▼

A method for covalent immobilization of a carbohydrate molecule with an oppositely charged surface, comprising the steps of adsorbing the carbohydrate molecule to the oppositely charged surface in proximity to reactive moieties bound to the charged surface; and next activating the bound moiety sufficiently for covalent attachment to the adsorbed carbohydrate molecule. Also disclosed is a hydrophobic microporous polymer membrane coated with a cross-linked, cationic polymer having fixed charges thereon, the coating having enhanced epoxide content for covalent immobilization of the oppositely-charged carbohydrate molecule. A novel method for sequencing carbohydrates by covalent attachment of a carbohydrate conjugate to the membrane, and subsequent treatment with glycosidases, is also presented.

대표청구항 ▼

A method for covalent immobilization of a charged carbohydrate conjugate with an oppositely charged surface, comprising the steps of: (a) coating a microporous pelymeric base membrane with a cationic polyamido-polyamine epichlorohydrin polymer having fixed charges thereon; (b) cross-linking said cationic polymer onto said polymeric base membrane by using less than a stoichiometric amount of base relative to the original epichlorohydrin content of said cationic polymer such that a composite membrane is formed which has sufficient epichlorohydrin and epoxide moieties remaining on the surface of said composite membrane for covalent immobilization of an oppositely-charged carbohydrate conjugate; (c) adsorbing said charged carbohydrate conjugate to said composite membrane in proximity to said epichlorohydrin and epoxide moieties bound to said composite membrane and being capable of reacting with said charged carbohydrate conjugate; and (d) activating said bound moieties sufficiently for covalent attachment to said adsorbed charged carbohydrate conjugate.

이 특허를 인용한 특허 (40)

Jones,Ronald M., Adhered membranes retaining porosity and biological activity.
상세보기
Etzel, Mark R., Adsorptive membranes for trapping viruses.
상세보기
Etzel, Mark R., Adsorptive membranes for trapping viruses.
상세보기
Bian, Nanying; Charkoudian, John; Soice, Neil; Umana, Joaquin; Wang, Chen, Affinity chromatography matrices and methods of making and using the same.
상세보기
Bian, Nanying; Charkoudian, John; Soice, Neil; Umana, Joaquin; Wang, Chen, Affinity chromatography matrices and methods of making and using the same.
상세보기
Bian, Nanying; Charkoudian, John; Soice, Neil; Umana, Joaquin; Wang, Chen, Affinity chromatography matrices and methods of making and using the same.
상세보기
Schweitzer,Markus; Windhab,Norbert; Havens,John R.; Onofrey,Thomas J.; Greef,Charles; Wang,Daguang, Biomolecules having multiple attachment moieties for binding to a substrate surface.
상세보기
Nishimi, Taisei; Ezoe, Toshihide; Kubo, Toshiaki; Tomita, Hidetoshi, Biosensor.
상세보기
Wang I-fan ; Zepf Robert, Cationically charge-modified membranes.
상세보기
Wang, I-fan; Zepf, Robert, Cationically charge-modified membranes.
상세보기
Wei, Ning; Lyng, Robert John; Varriale, Monica Graciela, Cationically charged coating on hydrophobic polymer fibers with poly (vinyl alcohol) assist.
상세보기
Wei, Ning; Lyng, Robert John; Varriale, Monica Graciela, Cationically charged coating on hydrophobic polymer fibers with poly (vinyl alcohol) assist.
상세보기
Wu, Xiaosong; Kinsey, Jr., Joe L.; Ishee, Michael; Konstantin, Peter; Shertok, Joel; Yang, Yujing, Charged membrane.
상세보기
Wu,Xiaosong; Kinsey, Jr.,Joe L.; Ishee,Michael; Konstantin,Peter; Shertok,Joel; Yang,Yujing, Charged membrane.
상세보기
Charkoudian, John; Soice, Neil P., Coated membranes and other articles.
상세보기
Khan, Shireen; Qian, Suyue; Shindelman, Jeff; Withers, III, George E.; Gee, Eileen; Chapman, Jr., William H.; Bennett, Greg; Schaal, Thomas D., Device and method for measuring LDL-associated cholesterol.
상세보기
Li, Fuming B.; Bai, Feng; Sebastian, John M.; Jones, Marvin E., Electret webs with charge-enhancing additives.
상세보기
Sebastian, John M.; Li, Fuming B.; Jones, Marvin E.; Dressel, Luke T.; Japuntich, Daniel A., Electret webs with charge-enhancing additives.
상세보기
Jones, Ronald M.; Worthy, Thomas E.; Nugent, Anthony J., High-density lipoprotein assay device and method.
상세보기
Jones, Ronald M.; Worthy, Thomas E.; Nugent, Anthony J., High-density lipoprotein assay device and method.
상세보기
Jones, Ronald M.; Worthy, Thomas E.; Shindelman, Jeffrey; Bellet, Neal F.; Nugent, Anthony J., High-density lipoprotein assay device and method.
상세보기
Raddatz,Stefan; M체ller Ibeler,Jochen; Schweitzer,Markus; Br체cher,Christoph; Windhab,Norbert; Havens,John R.; Onofrey,Thomas J.; Greef,Charles H.; Wang,Daguang, Hydrazide building blocks and hydrazide modified biomolecules.
상세보기
Williamson Bruce S. ; McGrath Kevin P., Integrated filter combining physical adsorption and electrokinetic adsorption.
상세보기
Sheikh-Ali, Bashir Musse, Leukocyte depletion filter media, filter produced therefrom, method of making same and method of using same.
상세보기
Tarbet Bryon J. ; Bruening Ronald L. ; Di Leo Anthony J. ; Goddard Philip M. ; Scarmoutzos Louis M., Method of making a composition for separating and concentrating certain ions from mixed ion solutions.
상세보기
Tarbet, Bryon J.; Bruening, Ronald L.; Di Leo, Anthony J.; Goddard, Philip M.; Scarmoutzos, Louis M., Method of making a composition for separating and concentrating certain ions from mixed ion solutions.
상세보기
Sebastian, John M.; Jones, Marvin E.; Li, Fuming B.; Chou, Shih-Hung; Schultz, Nathan E.; Keough, Justin L.; Shah, Rahul R.; Japuntich, Daniel A., Method of making electret articles based on zeta potential.
상세보기
Mitchell, Michael Donovan; Collias, Dimitris Ioannis; Bjorkquist, David William; Zaveri, Piyush Narendra; Woolley, Matthew Morgan, Methods for treating water.
상세보기
Mitchell, Michael Donovan; Collias, Dimitris Ioannis; Bjorkquist, David William; Zaveri, Piyush Narendra; Woolley, Matthew Morgan, Methods for treating water.
상세보기
Mitchell, Michael Donovan; Collias, Dimitris Ioannis; Bjorkquist, David William; Zaveri, Piyush Narendra; Woolley, Matthew Morgan, Methods of treating water.
상세보기
Hou, Kenneth C.; Bretl, Donald S.; Hembree, Richard D., Microorganism filter and method for removing microorganism from water.
상세보기
Weng, Ellen; Fonnum, Geir; Modahl, Grete Irene; Molteberg, Astrid Evenrød; Finne, Erling Sigurd; Stene, Torkel, Particles.
상세보기
Weng, Ellen; Fonnum, Geir; Modahl, Grete; Molteberg, Astrid; Finne, Erling, Particles.
상세보기
Weng, Ellen; Fonnum, Geir; Modahl, Grete; Molteberg, Astrid; Stene, Torkel, Particles.
상세보기
Koontz Steven L., Porous article with surface functionality and method for preparing same.
상세보기
Ruckenstein Eli ; Zeng Xianfang, Purification of wheat germ agglutinin using macroporous or microporous filtration membrane.
상세보기
Gordon II Joseph Grover ; Hart Mark Whitney ; Swanson Sally Ann, Stable electrophoretic particles for displays.
상세보기
Bahm, Jeannine Rebecca; Pearks, Andrew Thomas; Vidal, Guillermo Matias; Collias, Dimitris Ioannis; Mitchell, Michael Donovan; Astle, Robert E.; Faye, Katharine L. K.; Governal, Robert Andrew; Hamlin, Thomas J.; Lucht, Rebecca A.; Patel, Hemang, Water filter materials and water filters containing a mixture of microporous and mesoporous carbon particles.
상세보기
Bahm, Jeannine Rebecca; Pearks, Andrew Thomas; Vidal, Guillermo Matias; Collias, Dimitris Ioannis; Mitchell, Michael Donovan; Astle, Robert E.; Faye, Katharine L. K.; Governal, Robert Andrew; Hamlin, Thomas J.; Lucht, Rebecca A.; Patel, Hemang, Water filter materials and water filters containing a mixture of microporous and mesoporous carbon particles.
상세보기
Tanner, John D.; Emmons, David James; Riedel, Richard P.; Mitchell, Michael Donovan; Collias, Dimitris Ioannis, Water treating methods.
상세보기

IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표IPC 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 공고번호, 공고일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표출원인, 출원인국적, 출원인주소, 발명자, 발명자E, 발명자코드, 발명자주소, 발명자 우편번호, 발명자국적, 대표IPC, IPC코드, 요약, 미국특허분류, 대리인주소, 대리인코드, 대리인(한글), 대리인(영문), 국제공개일자, 국제공개번호, 국제출원일자, 국제출원번호, 우선권, 우선권주장일, 우선권국가, 우선권출원번호, 원출원일자, 원출원번호, 지정국, Citing Patents, Cited Patents
저장형식	Text(ASCII format) Excel format PIAS분석(.xls)
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Method and apparatus for covalent immobilization of charge- conjugated carbohydrate molecules 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

이 특허를 인용한 특허 (40)

관련 콘텐츠

특허 원문 보기

IPC 상위 출원인

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

Method and apparatus for covalent immobilization of charge- conjugated carbohydrate molecules 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

이 특허를 인용한 특허 (40)

관련 콘텐츠

특허 원문 보기

IPC 상위 출원인

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트