초록

Somatic embryo suspension cultures and pro-embryogenic mass suspension cultures (PEMs) comprising somatic embryos and PEMs having substantially similar ploidy levels, plants derived therefrom, and methods for obtaining said somatic embryos.

대표청구항 ▼

A method of obtaining somatic embryos from non-callus material in suspension culture which comprises: (i) culturing non-Daucus non-callus explant material in a liquid medium comprising an effective amount of auxin or mixture of auxins sufficient to promote the induction of pro-embryogenic mass (PEM)

A method of obtaining somatic embryos from non-callus material in suspension culture which comprises: (i) culturing non-Daucus non-callus explant material in a liquid medium comprising an effective amount of auxin or mixture of auxins sufficient to promote the induction of pro-embryogenic mass (PEM) formation; (ii) increasing the number of induced PEMs by cultivation of said PEMs in an auxin-containing liquid medium; (iii) collecting PEMs obtained in step (ii) and placing them in an auxin-free liquid medium to induce somatic embryo formation; (iv) inducing somatic embryo formation; and (v) collecting somatic embryos resulting from the induced embryos of step (iv) wherein the somatic embryos collected in step (v) have the same ploidy level as the non-callus starting material in suspension culture and said ploidy level is either diploid or tetraploid.