초록 ▼

Synthetic nucleic acid molecules are non-covalently immobilized in the presence of a salt or cationic detergent on a hydrophilic polystyrene solid support containing an －OH, －C〓O or －COOH hydrophilic group or on a glass solid support. The support is contacted with a solution having a pH of about 6 t

Synthetic nucleic acid molecules are non-covalently immobilized in the presence of a salt or cationic detergent on a hydrophilic polystyrene solid support containing an －OH, －C〓O or －COOH hydrophilic group or on a glass solid support. The support is contacted with a solution having a pH of about 6 to about 8 containing the synthetic nucleic acid and the cationic detergent or salt. Preferably, the cationic detergent is 1-ethyl-3-(3′-dimethylaminopropyl)-1,3-carbodiimide hyrochloride at a concentration of about 30 mM to about 100 mM or octyldimethylamine hydrochloride at a concentration of about 50 mM to about 150 mM. The salt is preferably NaCl at a concentration of about 50 mM to about 250 mM. When the detergent is 1-ethyl-3-(3′-dimethylaminopropyl)-1,3-carbodiimide hyrochloride, the glass support or the hydrophilic polystyrene support is used. When NaCl or octyldimethylamine hydrochloride is used, the support is the hydrophilic polystyrene. After immobilization, the support containing the immobilized nucleic acid may be washed with an aqueous solution containing a non-ionic detergent. The immobilized nucleic acid may be used in nucleic acid hybridization assays, nucleic acid sequencing and in analysis of genomic polymorphisms.

대표청구항 ▼

A method for non-covalently immobilizing a synthetic nucleic acid molecule on a solid support which is a hydrophilic polystyrene solid support containing a hydrophilic group selected from the group consisting of －OH, －C〓O, and －COOH, or a glass solid support, said method comprising the steps: (a) co

A method for non-covalently immobilizing a synthetic nucleic acid molecule on a solid support which is a hydrophilic polystyrene solid support containing a hydrophilic group selected from the group consisting of －OH, －C〓O, and －COOH, or a glass solid support, said method comprising the steps: (a) contacting said support with a solution having a pH of from about 6 to about 8, and containing said nucleic acid and (1) a cationic detergent selected from the group consisting of 1-ethyl-3-(3′-dimethylaminopropyl)-1,3-carbodiimide hydrochloride provided at a concentration of from about 30 mM to about 100 mM, and octyldimethylamine hydrochloride provided at a concentration of from about 50 mM to about 150 mM or (2) NaCl provided at a concentration of from about 50 mM to about 250 mM, to thereby non-covalently immobilize said nucleic acid to said support, wherein: (i) when said cationic detergent is 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide-1,3-hydrochloride, said support is selected from the group consisting of glass or said hydrophilic polystyrene; (ii) when said cationic detergent is octyldimethylamine hydrochloride, said support is said hydrophilic polystyrene; and (iii) when said solution contains said NaCl, said support is said hydrophilic polystyrene; and (b) subsequently washing said solid support with an aqueous solution.