초록 ▼

A method for detecting the presence of antibody to FIV in a biological sample. The method includes the steps of providing a first antigen having a first epitope recognized by the antibody, the first antigen being detectable; contacting the first antigen with the sample under conditions under which t

A method for detecting the presence of antibody to FIV in a biological sample. The method includes the steps of providing a first antigen having a first epitope recognized by the antibody, the first antigen being detectable; contacting the first antigen with the sample under conditions under which the antibody can bind to the first antigen to form an immune complex; and detecting the immune complex. An ELISA test for FIV, using purified FIV antigen, is also described.

대표청구항 ▼

A method for detecting an antibody and an antigen in a single biological sample, said method comprising: (a) providing an antigen which selectively forms a first immune complex with a sample antibody, said antigen being directly bound to a solid support at a first location; (b) providing an antibody

A method for detecting an antibody and an antigen in a single biological sample, said method comprising: (a) providing an antigen which selectively forms a first immune complex with a sample antibody, said antigen being directly bound to a solid support at a first location; (b) providing an antibody which selectively forms a second immune complex with a sample antigen, said antibody being directly bound to said solid support at a second location; (c) contacting said first location on said solid support with at least a portion of a biological sample under conditions whereby said first immune complex can form and contacting said second location on said solid support with at least a portion of said sample under conditions whereby said second immune complex can form; (d) washing unbound material from said first location and from said second location; and (e) separately detecting whether said first immune complex is formed and whether said second immune complex is formed, said first immune complex being detected with a labelled antigen which selectively binds to said first complex and said second immune complex being detected by adding a labelled antibody which selectively binds to said second complex, said labelled antibody being presented to both said first and said second locations.