초록 ▼

A blood sample is gravimetrically separated into its heavier and lighter phases by centrifugation in a tube. The phases are separated by a disc which has a center of buoyancy and a center of mass that are spaced apart from each other along the axis of symmetry of the disc. The diameter of the disc i

A blood sample is gravimetrically separated into its heavier and lighter phases by centrifugation in a tube. The phases are separated by a disc which has a center of buoyancy and a center of mass that are spaced apart from each other along the axis of symmetry of the disc. The diameter of the disc is slightly larger than the bore diameter of the tube. The disc is initially positioned in the tube with its axis of symmetry perpendicular to the axis of the tube so that blood drawn into the tube can readily pass by the disc. Upon centrifugation, the disc will tumble through a 90°angle so that the axis of symmetry of the disc will be coincidental with the axis of the tube. Once the disc has tumbled in the sample, the ongoing centrifugal forces will cause a stretching of the disc along its axis of symmetry thereby decreasing the diameter of the disc. The stretching forces exerted on the disc will temporarily decrease the effective specific gravity of the disc so that the disc will remain in the upper portion of the lighter phase of the sample during the initial portion of the centrifugation. As the centrifuge slows down, the disc sinks through the lighter phase of the sample and settles on the interface between the lighter and heavier phases of the sample, thereby separating the two phases of the sample. One of the phases can then be removed from the tube without contamination from the other phase.

대표청구항 ▼

A separator body for separating a formed phase from an unformed phase in a centrifuged sample of a multi-phase material in a test tube, said separator body being sized to fit snugly in said test tube and being generally disc-shaped and having an axis of symmetry which is disposed perpendicular to it

A separator body for separating a formed phase from an unformed phase in a centrifuged sample of a multi-phase material in a test tube, said separator body being sized to fit snugly in said test tube and being generally disc-shaped and having an axis of symmetry which is disposed perpendicular to its circumference, and said separator body having a center of mass (Cm) and a center of buoyancy (Cb) which are spaced apart from each along said axis of symmetry of the body so as to impart to the body a first side having a higher specific gravity, and a second opposite side having a lower specific gravity. A method for gravimetrically separating a formed phase from an unformed phase in a centrifuged sample of a mult-phase material in an assembly which comprises a tube having a bore with an axis of elongation, a generally disc-shaped separator body having an axis of symmetry which is disposed perpendicular to its circumference and having a center of mass (Cm) and a center of buoyancy (Cb) which are spaced apart from each along said axis of symmetry so as to impart to the body a first side having a higher specific gravity, and a second opposite side having a lower specific gravity, which body is positioned in said tube bore with the axis of symmetry of said body being disposed at essentially a 90°angle relative to the axis of elongation of the tube bore so as to open the tube bore for admission of the sample into the tube, said method comprising the steps of: a) drawing the sample of the multi-phase material into the tube; b) centrifuging the multi-phase material sample at G forces which will separate the formed phase from the unformed phase and will cause the separator body to tumble in the tube to a barrier position wherein the axis of symmetry of the separator body coincides with the axis of elongation of the tube bore; and c) causing the separator body to gravitate onto an interface between the formed and unformed phases of the material sample during said centrifuging step so as to form a barrier between the formed and unformed phases in the sample.