초록 ▼

Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system furthe

Disclosed are devices for detecting the presence of a preselected analyte in a fluid sample. The devices comprise a substrate microfabricated to define a sample inlet port, and a mesoscale flow system that includes a sample flow channel extending from the inlet port. The mesoscale flow system further includes an analyte detection region in fluid communication with the flow channel comprised of a binding moiety for specifically binding the analyte. The detection region is constructed with a mesoscale dimension sufficiently small to enhance binding of the binding moiety and the analyte. The binding moiety may be immobilized in the detection region. The mesoscale detection systems of the invention may be used in a wide range of applications, including the detection of cells or macromolecules, or for monitoring reactions or cell culture growth.

대표청구항 ▼

A device for detecting the presence of an analyte in a fluid sample, the device comprising: a solid substrate microfabricated to define: a sample inlet port, said port being dimensioned for connection to a pump means; and a mesoscale flow system comprising: a sample flow channel extending from said

A device for detecting the presence of an analyte in a fluid sample, the device comprising: a solid substrate microfabricated to define: a sample inlet port, said port being dimensioned for connection to a pump means; and a mesoscale flow system comprising: a sample flow channel extending from said inlet port; and an analyte binding region in fluid communication with said flow channel comprising a binding moiety immobilized on a surface in said analyte binding region, wherein said binding moiety specifically binds to analyte in said fluid sample, at least one of said sample flow channel or said analyte binding region having mesoscale cross-sectional dimensions; and a binding means associated with said analyte binding region or in fluid communication with said analyte binding region for detecting binding of said analyte to said binding moiety.