[특허]In situ PCR amplification system

In situ PCR amplification system 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12M-001/40 C12M-001/34
출원번호	US-0525321 (1995-09-08)
발명자 / 주소	Atwood John G. (West Redding CT) Picozza Enrico (Newtown CT)
출원인 / 주소	The Perkin-Elmer Corporation (Norwalk CT 02)
인용정보	피인용 횟수 : 48 인용 특허 : 0

초록 ▼

A complete in situ PCR system for amplification of nucleic acids contained in a prepared cell or tissue sample. The containment system for the PCR sample comprises a glass microscope slide, a specimen sample containing the target nucleic acid sequence mounted on the slide, a flexible plastic cover over the sample, and a retaining assembly fastened to the slide and to the cover to retain and seal a reaction mixture against the sample during thermal cycling. The retaining assembly includes a rigid ring on a rim portion of the cover, a cross beam having spaced parallel rails joined by opposite flat ends, and a pair of clips which are pressed over the ends and opposite sides of the slide to fasten the cross beam and cover to the slide. In one form the retaining assembly includes a flexible compliant cover member, which is permeable to gas but non-permeable to liquid, for forming a closed liquid space adjacent a flat surface, a cross beam member enclosing the cover member to form a closed gas space therebetween, the periphery of the liquid space and of the gas space being sealed, and a pressure differential being established whereby the pressure in the liquid space is greater than the pressure in the gas space.

대표청구항 ▼

A retaining assembly for retaining a liquid against a substantially flat surface so that said flat surface defines a boundary of said liquid, said assembly comprising, in combination, means for forming a closed liquid space adjacent said flat surface, said means for forming a closed liquid space including a flexible compliant cover member, said cover member being permeable to gas and non-permeable to liquid, a cross beam member enclosing said cover member to form a gas space there between, means for sealing the periphery of said liquid space and for sealing the periphery of said gas space, and means for establishing a pressure differential between said liquid space and said gas space, the pressure in said liquid space being greater than the pressure in said gas space. A retaining assembly for retaining liquid against a slide comprising a flexible compliant cover member having an annular rim portion, a cross beam member completely enclosing said cover member and having an annular ring portion for engaging said annular rim portion, and at least one clip means for removably retaining said ring portion in sealed engagement with said rim portion and said rim portion in sealed engagement with said slide.

이 특허를 인용한 특허 (48)

Brown, Larry R.; Brumley, William D.; Zajac, Kenneth J., Apparatus and method for flexible heating cover assembly for thermal cycling of samples of biological material.
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Kram, Brian Howard; Marshall, Kevin David; Otter, Michael; Martin, Carl David; Jones, Jessica, Arcuate member for moving liquids along a microscope slide.
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Kram, Brian Howard; Marshall, Kevin David; Otter, Michael; Martin, Carl David; Jones, Jessica, Arcuate member for moving liquids along a microscope slide.
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Eastman, Jay M.; Fox, William J.; Greenwald, Roger J.; Roesser, Kevin P.; Zavislan, James M., Cassette for facilitating optical sectioning of a retained tissue specimen.
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Eastman, Jay M.; Fox, William J.; Greenwald, Roger J.; Roesser, Kevin P.; Zavislan, James M., Cassette for facilitating optical sectioning of a retained tissue specimen.
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Cosby, N. Guy; Moore, David J.; Clements, Jim, Cover slip mixing apparatus.
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Becker,Horst Dieter; Einsle,Xaver, Device and method for wetting objects.
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Klaus W. Berndt, Device for preparing thin liquid for microscopic analysis.
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Kreifels, Matthew R.; Whitney, Scott E.; Wilder, Gregg; Flanagan, John; Dowling, James; Hills, Philip; Tilleman, Michael; Jackson, Jeremie, Devices for real-time polymerase chain reaction.
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Rong, Guangwei; Duan, Pingsen; Yang, Qing; Han, Weifeng, Fixing device for heat exchanger.
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Lyman, George F.; Myer, Vic E.; Wilson, Christopher J., Hybridization chamber for high density nucleic acid arrays.
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Hsieh,Huangpin Ben; Kneissl,Michael A., LED or laser enabled real-time PCR system and spectrophotometer.
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Adey,Nils B., Laminated microarray interface device.
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Baer, Thomas M.; Reamey, Robert H.; Moravick, Keith E.; Head, David F.; Richardson, Bruce J.; Richardson, Derrick A., Laser capture microdissection (LCM) extraction device and device carrier, and method for post-LCM fluid processing.
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Baer, Thomas M.; Reamey, Robert H.; Moravick, Keith E.; Head, David F.; Richardson, Bruce J.; Richardson, Derrick A., Laser capture microdissection (LCM) extraction device and device carrier, and method for post-LCM fluid processing.
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Tsipouras, Petros; Tafas, Triantafyllos, Method and apparatus for computer controlled rare cell, including fetal cell, based diagnosis.
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Tsipouras, Petros; Tafas, Triantafyllos, Method and apparatus for computer controlled rare cell, including fetal cell, based diagnosis.
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Brown,Larry R.; Brumley,William D.; Zajac,Kenneth J., Method and apparatus for cover assembly for thermal cycling of samples.
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Mack J. Schermer ; C. Todd Lombardo ; Robert Milkowski ; Donald Regan ; Paul St. Cyr, Method and apparatus for incubation of a liquid reagent and target spots on a microarray substrate.
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Kram, Brian H., Method and apparatus for treating a biological sample with a liquid reagent.
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McNeely,Michael; Adey,Nils; Spute,Mark; Ayliffe,Edward; Howard,Michael; Beutel,Darin; Jensen,John; Coffin,Stephen; Moyer,Thomas, Method and system for microfluidic interfacing to arrays.
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Schembri,Carol T.; Overman,Leslie B.; Hotz,Charles Z., Method for conducting binding reactions on a solid surface within an enclosed chamber.
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Schembri, Carol T.; Overman, Leslie B.; Hotz, Charles Z., Method for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber.
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Berndt Klaus W., Method for preparing thin liquid samples for microscopic analysis.
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Chesnut, Jonathan D.; Carrino, John; Leong, Louis; Madden, Knut; Gleeson, Martin; Fan, James; Brasch, Michael; Cheo, David; Hartley, James L.; Byrd, Devon R.; Temple, Gray F., Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites.
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Chesnut, Jonathan; Carrino, John; Leong, Louis; Madden, Knut; Gleeson, Martin; Fan, James; Brasch, Michael; Cheo, David; Hartley, James; Byrd, Devon; Temple, Gary, Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites.
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Chesnut, Jonathan; Carrino, John; Leong, Louis; Madden, Knut; Gleeson, Martin; Fan, James; Brasch, Michael; Cheo, David; Hartley, James; Byrd, Devon; Temple, Gary, Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites.
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Tan, Eugene; Selden, Richard F; Turingan, Rosemary S., Methods for forensic DNA quantitation.
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Selden, Richard F.; Tan, Eugene; Lam, Heung Chuan; Giese, Heidi Susanne; Kellogg, Gregory John; Wright, John A., Methods for rapid multiplexed amplification of target nucleic acids.
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Selden, Richard F.; Tan, Eugene; Lam, Heung Chuan; Giese, Heidi Susanne; Kellogg, Gregory John; Wright, John A., Methods for rapid multiplexed amplification of target nucleic acids.
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Cutler Thomas A. ; Lalonde Guy ; Kelly Andrew J. G. ; Wagstrom Christopher R., Nano-grid micro reactor and methods.
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Chesnut, Jon; Leong, Louis, Nucleic acid molecules containing recombination sites and methods of using the same.
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Chesnut, Jonathan; Leong, Louis, Nucleic acid molecules containing recombination sites and methods of using the same.
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Otter, Michael; Kram, Brian Howard; Martin, Carl David; Jones, Jessica; Marshall, Kevin David; Tse, Christine; Harrison, Josh, Opposables and automated specimen processing systems with opposables.
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Weight, Brent L.; Bickmore, William D.; Zitting, Darryl K., Pressurizable cartridge for polymerase chain reactions.
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TerMaat, Joel; Whitney, Scott E.; Viljoen, Hendrik J.; Kreifels, Matthew R., Rapid thermocycler system for rapid amplification of nucleic acids and related methods.
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Lee, Martin Alan; Squirrell, David James; Jones, Ross Peter; Williamson, Roger James; Gregory, George Richard; Gutsell, Graham, Reaction vessel comprising conductive layer and inner non-metallic layer.
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Kreifels, Matthew R.; Whitney, Scott E.; TerMaat, Joel R., Real-time optical system for polymerase chain reaction.
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Eastman, Jay M.; Fox, William J.; Greenwald, Roger J.; Roesser, Kevin P.; Zavislan, James M., System for optically sectioning a tissue specimen.
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Toh, Tiong Han; Loh, Wuh Ken; Boo, Kuan Moon; Klein, Sandro; Welsh, Daniel, Thermal cycler cover.
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Termaat, Joel R.; Viljoen, Hendrik J.; Whitney, Scott E., Thermocycler and sample vessel for rapid amplification of DNA.
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Kram, Brian Howard; Marshall, Kevin David; Tse, Christine; Keller, Timothy James; Ashby, Austin Micheil, Thin film processing apparatuses for adjustable volume accommodation.
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Kram, Brian Howard; Marshall, Kevin David; Tse, Christine; Keller, Timothy James; Ashby, Austin Micheil, Thin film processing apparatuses for adjustable volume accommodation.
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Greenwald, Roger J.; Zavislan, James M., Tissue specimen holder.
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Fox, William J.; DiStasio, Christopher C., Tissue specimen stage for an optical sectioning microscope.
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Yuen, Po Ki, Well frame including connectors for biological fluids.
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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표IPC 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 공고번호, 공고일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표출원인, 출원인국적, 출원인주소, 발명자, 발명자E, 발명자코드, 발명자주소, 발명자 우편번호, 발명자국적, 대표IPC, IPC코드, 요약, 미국특허분류, 대리인주소, 대리인코드, 대리인(한글), 대리인(영문), 국제공개일자, 국제공개번호, 국제출원일자, 국제출원번호, 우선권, 우선권주장일, 우선권국가, 우선권출원번호, 원출원일자, 원출원번호, 지정국, Citing Patents, Cited Patents
저장형식	Text(ASCII format) Excel format PIAS분석(.xls)
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
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연합인증

In situ PCR amplification system 원문보기

초록 ▼

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이 특허를 인용한 특허 (48)

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연합인증

In situ PCR amplification system 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

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이 특허를 인용한 특허 (48)

관련 콘텐츠

특허 원문 보기

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