초록 ▼

To detect bands in an electrophoresis capillary tube having a liquid separating medium, a light source and a light detector of a monitor are positioned on opposite sides of the capillary tube with the light source transmitting light through a first slit and light passing to the detector through a se

To detect bands in an electrophoresis capillary tube having a liquid separating medium, a light source and a light detector of a monitor are positioned on opposite sides of the capillary tube with the light source transmitting light through a first slit and light passing to the detector through a second slit after it has passed through the capillary tube. The first and second slits are aligned with the direction of motion of bands, have a maximum length of less than 500 micrometers and a maximum width of less than 200 micrometers. There are no vertical lengths in the capillary tube having a dimension greater than one third of its length between a sample injecting end and a detecting end of the capillary tube. A sample injector includes a vacuum chamber communicating with one end of the capillary tube to pull sample into the other end and a pressure sensor to measure the pressure in said vacuum chamber and generate a signal indicative thereof and integrate it to indicate the amount of sample pulled into the system.

대표청구항 ▼

A method of performing electrophoresis comprising the steps of: positioning a preselected length of capillary tube containing a separation medium in a support, with the capillary tube being coiled to fit a fixed area of the support wherein different lengths of capillary tubes may be placed in the sa

A method of performing electrophoresis comprising the steps of: positioning a preselected length of capillary tube containing a separation medium in a support, with the capillary tube being coiled to fit a fixed area of the support wherein different lengths of capillary tubes may be placed in the same support; mounting said support horizontally between a location for injection of samples and a location for the sensing of bands wherein the capillary tube is sufficiently horizontal between the location for injection of samples and the location for sensing bands to have no vertical lengths having a dimension greater than one third of the distance between the location for injecting samples and the location for sensing bands; establishing a potential across the separating medium; introducing a sample into the separating medium for electrophoresis through it; using a potential sufficiently high to cause convection currents within said separating medium in a substantially horizontal portion of said separating medium under conditions in which there are no significant bands of sample being separated in a region with a substantially vertical component capable of causing band spreading by convection in the direction of migration.