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Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C12Q-001/68
  • C12N-015/09
  • C12N-015/10
출원번호 US-0485606 (1995-06-07)
발명자 / 주소
  • Sapolsky Ronald J.
  • Lipshutz Robert J.
  • Gingeras Thomas R.
출원인 / 주소
  • Affymetrix, Inc.
대리인 / 주소
    Townsend & Townsend & Crew LLP
인용정보 피인용 횟수 : 125  인용 특허 : 4

초록 ▼

The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the "capturing" and determination of specific oligonucleotide sequences located adjacent to Type-IIs

대표청구항 ▼

[ What is claimed is:] [1.] A method of identifying sequences in a polynucleotide sequence, comprising:(a) cleaving the polynucleotide sequence with a first type-IIs endonuclease;(b) ligating a first adapter sequence to the polynucleotide sequence cleaved in step (a), said first adapter having a rec

이 특허에 인용된 특허 (4)

  1. Deugau Kenneth V. (Kingston CAX) Unrau Paul (Deep River CAX), Indexing linkers.
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  2. Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA), Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof.
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  3. Drmanac Radoje T. (Zvecanska 46 Beograd 11000) Crkvenjakov Radomir B. (Bulevar JNA 118 Beograd YUX 11000), Method of sequencing of genomes by hybridization of oligonucleotide probes.
    상세보기
  4. Macevicz Stephen C. (21890 Rucker Dr. Cupertino CA 95014), Nucleic acid sequence determination by multiple mixed oligonucleotide probes.
    상세보기

이 특허를 인용한 특허 (125)

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