초록 ▼

A method is provided for detecting nucleic acid sequence aberrations using two immobilization steps. According to the method, a nucleic acid sequence aberration is detected by detecting nucleic acid sequences having both a first nucleic acid sequence type (e.g., from a first chromosome) and a second

A method is provided for detecting nucleic acid sequence aberrations using two immobilization steps. According to the method, a nucleic acid sequence aberration is detected by detecting nucleic acid sequences having both a first nucleic acid sequence type (e.g., from a first chromosome) and a second nucleic acid sequence type (e.g., from a second chromosome), the presence of the first and the second nucleic acid sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. In the method, immobilization of a first hybridization probe is used to isolate a first set of nucleic acids in the sample which contain the first nucleic acid sequence type. Immobilization of a second hybridization probe is then used to isolate a second set of nucleic acids from within the first set of nucleic acids which contain the second nucleic acid sequence type. The second set of nucleic acids are then detected, their presence indicating the presence of a nucleic acid sequence aberration.

대표청구항 ▼

[ What is claimed is:] [1.] A method for detecting nucleic acid aberrations in a sample of chromosomal DNA characterized by a rearrangement which forms a nucleic acid sequence which includes a first sequence unique to a first region of a chromosome, a second sequence unique to a second, different re

[ What is claimed is:] [1.] A method for detecting nucleic acid aberrations in a sample of chromosomal DNA characterized by a rearrangement which forms a nucleic acid sequence which includes a first sequence unique to a first region of a chromosome, a second sequence unique to a second, different region of the same chromosome or a different chromosome and a region connecting the first and second regions which includes a site of the rearrangement, the method comprising:a) contacting a first hybridization probe which includes a nucleic acid sequence complementary to the first region with a sample of chromosomal DNA under conditions favorable for hybridization such that the first hybridization probe hybridizes to a first set of chromosomal DNA which include a nucleic acid sequence from the first region;b) immobilizing the first hybridization probe by forming a linkage attaching the first hybridization probe to a first solid support;c) after performing steps a-b in any order, releasing the first set of chromosomal DNA from the first solid support;d) contacting the first set of chromosomal DNA under conditions favorable for hybridization with a second hybridization probe which includes a nucleic acid sequence complementary to the second region, the second hybridization probe hybridizing to a second set of chromosomal DNA which include those chromosomal DNA of the first set which include a nucleic acid sequence from the second region;e) immobilizing the second hybridization probe by forming a linkage attaching the second hybridization probe to a second solid support, thereby immobilizing the second set of chromosomal DNA; andf) detecting the second set of chromosomal DNA immobilized on the second solid support, the presence of the second set of chromosomal DNA indicating the presence of a nucleic acid sequence aberration without indicating the sequence of the region connecting the first and second regions.