초록 ▼

A method and a device for separating a component, such as fibrin monomer from blood, by centrifugation, involve feeding of blood admixed an anticoagulant to a first annular chamber in a device, where the annular chamber is defined by a cylindrical outer wall and a cylindrical inner wall, both walls

A method and a device for separating a component, such as fibrin monomer from blood, by centrifugation, involve feeding of blood admixed an anticoagulant to a first annular chamber in a device, where the annular chamber is defined by a cylindrical outer wall and a cylindrical inner wall, both walls extending coaxially about a common axis, as well as by a top wall and a bottom wall. The top wall or the bottom wall is formed by a piston body displaceable within the first chamber. This method involves furthermore a centrifugation of the device about the said common axis to substantially separate blood into a cell fraction and a plasma fraction followed by the resulting plasma fraction being transferred while influenced by the piston body to a second chamber defined by an outer cylindrical wall. The outer cylindrical wall extends coaxially with the said common axis, whereby a fraction with fibrin I is caused to be separated in the second chamber while a suitable enzyme is being added. The separation of fibrin monomer from the plasma fraction in the second chamber is carried out during continued centrifugation whereby a fibrin polymer is deposited on the cylindrical outer wall of said second chamber, whereafter the fluid fraction collected at the bottom of the second chamber is transferred while influenced by the piston body to the first chamber. The fraction with fibrin polymer remaining in the second chamber is substantially deposited on the cylindrical wall and is caused to be dissolved by addition of a solvent and by centrifugation, whereafter it is transferred to a receiving container by passing after addition of a binder to the enzyme through a filter removing said enzyme, whereby a fibrin monomer containing solution is provided.

대표청구항 ▼

[ We claim:] [1.] A device for separating a blood component from blood or plasma comprisinga reaction chamber defined by an outer wall and a bottom wall adapted to receive, and for centrifugation of, said blood or plasma; means for introducing into said reaction chamber an agent capable of promoting

[ We claim:] [1.] A device for separating a blood component from blood or plasma comprisinga reaction chamber defined by an outer wall and a bottom wall adapted to receive, and for centrifugation of, said blood or plasma; means for introducing into said reaction chamber an agent capable of promoting the separation of said blood component from said blood or plasma;means for centrifuging said reaction chamber, with said blood or plasma and agent therein sufficiently so as to separate said blood component from said blood or plasma and deposit said blood component within said reaction chamber; and transfer means comprising one or more fluid transfer channels extending through said outer wall such that blood or plasma can be transferred into or out of said reaction chamber.