초록 ▼

Method and apparatus for producing combinatorial position-addressable libraries of different-sequence oligomers or different-substituent small molecule compounds are disclosed. The method employs massive parallel synthesis by stepwise subunit addition or substituent addition in a dense capillary-tub

Method and apparatus for producing combinatorial position-addressable libraries of different-sequence oligomers or different-substituent small molecule compounds are disclosed. The method employs massive parallel synthesis by stepwise subunit addition or substituent addition in a dense capillary-tube array. The libraries allow high throughput screening of library compounds in either solid phase or solution phase, and position-related identification of active library compounds.

대표청구항 ▼

[ It is claimed:] [1.] A method of producing a position-addressable combinatorial library of different-sequence oligomer or different-substitiuent small molecule compounds, comprising the steps of:(i) identifying, in an array of capillary tubes, a selected subset of tubes into which a selected one o

[ It is claimed:] [1.] A method of producing a position-addressable combinatorial library of different-sequence oligomer or different-substitiuent small molecule compounds, comprising the steps of:(i) identifying, in an array of capillary tubes, a selected subset of tubes into which a selected one of a plurality of different chemical reagents in a reagent solution is to be introduced, and a complementary subset of remaining tubes in the array,(ii) selectively plugging a tube end of each capillary tube in said complementary subset,(iii) placing ends of the tubes in said array in contact with said solution, under conditions effective to draw solution into the unplugged tubes only,(iv) allowing the solution in the tubes in the selected subset to react with a wall portion of each tube on which a library compound is being formed,(v) unplugging the plugged tubes, and(vi) repeating steps (i)-(v) for different subsets of tubes and reagent solutions until said library is produced in said tubes.